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Carbon Dioxide Mediates the Response to Temperature and Water Activity Levels in Aspergillus flavus during Infection of Maize Kernels

1
USDA/Agricultural Research Service, 1100 Robert E Lee Blvd., New Orleans, LA 70124, USA
2
Applied Mycology Group, Cranfield Soil and Agrifood Institute, Cranfield University, Bedfordshire K43 0AL, UK
3
Food Hygiene and Safety, Meat and Meat products Research Institute, University of Extremadura, 10003 Caceres, Spain
4
Department of Entomology and Plant Pathology, 223 Partners III, P.O. Box 7567, North Carolina State University, Raleigh, NC 27695, USA
*
Author to whom correspondence should be addressed.
Received: 26 September 2017 / Revised: 17 November 2017 / Accepted: 14 December 2017 / Published: 22 December 2017
(This article belongs to the Collection Aflatoxins)
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Abstract

Aspergillus flavus is a saprophytic fungus that may colonize several important crops, including cotton, maize, peanuts and tree nuts. Concomitant with A. flavus colonization is its potential to secrete mycotoxins, of which the most prominent is aflatoxin. Temperature, water activity (aw) and carbon dioxide (CO2) are three environmental factors shown to influence the fungus-plant interaction, which are predicted to undergo significant changes in the next century. In this study, we used RNA sequencing to better understand the transcriptomic response of the fungus to aw, temperature, and elevated CO2 levels. We demonstrate that aflatoxin (AFB1) production on maize grain was altered by water availability, temperature and CO2. RNA-Sequencing data indicated that several genes, and in particular those involved in the biosynthesis of secondary metabolites, exhibit different responses to water availability or temperature stress depending on the atmospheric CO2 content. Other gene categories affected by CO2 levels alone (350 ppm vs. 1000 ppm at 30 °C/0.99 aw), included amino acid metabolism and folate biosynthesis. Finally, we identified two gene networks significantly influenced by changes in CO2 levels that contain several genes related to cellular replication and transcription. These results demonstrate that changes in atmospheric CO2 under climate change scenarios greatly influences the response of A. flavus to water and temperature when colonizing maize grain. View Full-Text
Keywords: aflatoxin; Zea mays; climate change; secondary metabolites; RNA-seq aflatoxin; Zea mays; climate change; secondary metabolites; RNA-seq
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Gilbert, M.K.; Medina, A.; Mack, B.M.; Lebar, M.D.; Rodríguez, A.; Bhatnagar, D.; Magan, N.; Obrian, G.; Payne, G. Carbon Dioxide Mediates the Response to Temperature and Water Activity Levels in Aspergillus flavus during Infection of Maize Kernels. Toxins 2018, 10, 5.

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