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Nutrients 2017, 9(9), 953; doi:10.3390/nu9090953

Effects of Simulated Human Gastrointestinal Digestion of Two Purple-Fleshed Potato Cultivars on Anthocyanin Composition and Cytotoxicity in Colonic Cancer and Non-Tumorigenic Cells

1
School of Human Nutrition, McGill University, 21,111 Lakeshore, Ste. Anne de Bellevue, QC H9X 3V9, Canada
2
Chemistry Department, Université du Québec à Montréal, P.O. Box 8888, Downtown Station, Montreal, QC H3C 3P8, Canada
3
BioMedical Engineering Department, McGill University, 3775 University Street, Room 311, Montreal, QC H3A 2B4, Canada
4
International Potato Center (CIP), Avenida La Molina 1895, Apartado Postal 1558, Lima, Lima 12, Peru
*
Authors to whom correspondence should be addressed.
Received: 22 June 2017 / Revised: 15 August 2017 / Accepted: 24 August 2017 / Published: 29 August 2017
(This article belongs to the Special Issue Effects of Polyphenol-Rich Foods on Human Health)
View Full-Text   |   Download PDF [2534 KB, uploaded 30 August 2017]   |  

Abstract

A dynamic human gastrointestinal (GI) model was used to digest cooked tubers from purple-fleshed Amachi and Leona potato cultivars to study anthocyanin biotransformation in the stomach, small intestine and colonic vessels. Colonic Caco-2 cancer cells and non-tumorigenic colonic CCD-112CoN cells were tested for cytotoxicity and cell viability after 24 h exposure to colonic fecal water (FW) digests (0%, 10%, 25%, 75% and 100% FW in culture media). After 24 h digestion, liquid chromatography-mass spectrometry identified 36 and 15 anthocyanin species throughout the GI vessels for Amachi and Leona, respectively. The total anthocyanin concentration was over thirty-fold higher in Amachi compared to Leona digests but seven-fold higher anthocyanin concentrations were noted for Leona versus Amachi in descending colon digests. Leona FW showed greater potency to induce cytotoxicity and decrease viability of Caco-2 cells than observed with FW from Amachi. Amachi FW at 100% caused cytotoxicity in non-tumorigenic cells while FW from Leona showed no effect. The present findings indicate major variations in the pattern of anthocyanin breakdown and release during digestion of purple-fleshed cultivars. The differing microbial anthocyanin metabolite profiles in colonic vessels between cultivars could play a significant role in the impact of FW toxicity on tumor and non-tumorigenic cells. View Full-Text
Keywords: purple-fleshed potato; anthocyanins; biotransformation; human gastrointestinal model; antioxidant; cancer cells; cytotoxicity purple-fleshed potato; anthocyanins; biotransformation; human gastrointestinal model; antioxidant; cancer cells; cytotoxicity
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Kubow, S.; Iskandar, M.M.; Melgar-Bermudez, E.; Sleno, L.; Sabally, K.; Azadi, B.; How, E.; Prakash, S.; Burgos, G.; Felde, T. Effects of Simulated Human Gastrointestinal Digestion of Two Purple-Fleshed Potato Cultivars on Anthocyanin Composition and Cytotoxicity in Colonic Cancer and Non-Tumorigenic Cells. Nutrients 2017, 9, 953.

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