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Nutrients 2017, 9(8), 883; doi:10.3390/nu9080883

Inhibitory Effect of Lycopene on Amyloid-β-Induced Apoptosis in Neuronal Cells

Department of Food and Nutrition, Brain Korea 21 PLUS Project, College of Human Ecology, Yonsei University, Seoul 03722, Korea
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Received: 12 June 2017 / Revised: 17 July 2017 / Accepted: 11 August 2017 / Published: 16 August 2017
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Abstract

Alzheimer′s disease (AD) is a fatal neurodegenerative disease. Brain amyloid-β deposition is a crucial feature of AD, causing neuronal cell death by inducing oxidative damage. Reactive oxygen species (ROS) activate NF-κB, which induces expression of Nucling. Nucling is a pro-apoptotic factor recruiting the apoptosome complex. Lycopene is an antioxidant protecting from oxidative stress-induced cell damage. We investigated whether lycopene inhibits amyloid-β-stimulated apoptosis through reducing ROS and inhibiting mitochondrial dysfunction and NF-κB-mediated Nucling expression in neuronal SH-SY5Y cells. We prepared cells transfected with siRNA for Nucling or nontargeting control siRNA to determine the role of Nucling in amyloid-β-induced apoptosis. The amyloid-β increased intracellular and mitochondrial ROS levels, apoptotic indices (p53, Bax/Bcl-2 ratio, caspase-3 cleavage), NF-kB activation and Nucling expression, while cell viability, mitochondrial membrane potential, and oxygen consumption rate decreased in SH-SY5Y cells. Lycopene inhibited these amyloid-β-induced alterations. However, amyloid-β did not induce apoptosis, determined by cell viability and apoptotic indices (p53, Bax/Bcl-2 ratio, caspase-3 cleavage), in the cells transfected with siRNA for Nucling. Lycopene inhibited apoptosis by reducing ROS, and by inhibiting mitochondrial dysfunction and NF-κB-target gene Nucling expression in neuronal cells. Lycopene may be beneficial for preventing oxidative stress-mediated neuronal death in patients with neurodegeneration. View Full-Text
Keywords: amyloid-β; apoptosis; NF-κB; Nucling; lycopene; reactive oxygen species amyloid-β; apoptosis; NF-κB; Nucling; lycopene; reactive oxygen species
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Hwang, S.; Lim, J.W.; Kim, H. Inhibitory Effect of Lycopene on Amyloid-β-Induced Apoptosis in Neuronal Cells. Nutrients 2017, 9, 883.

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