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Nutrients 2017, 9(8), 798; doi:10.3390/nu9080798

Sechium edule (Jacq.) Swartz, a New Cultivar with Antiproliferative Potential in a Human Cervical Cancer HeLa Cell Line

1
Postgraduate College, Campus Montecillo, Km 36.5 Mexico-Texcoco Highway, C.P. 56230 Texcoco, Mexico
2
Interdisciplinary Research Group of Sechium edule in Mexico (GISeM), Texcoco, Agustín Melgar 10 Street, C.P. 56153 Texcoco, Mexico
3
Postgraduate College, Campus San Luis Potosí, Iturbide No. 73 Street, Salinas de Hidalgo, C.P. 78600 San Luis Potosí, Mexico
4
FES Zaragoza, National Autonomous University of Mexico, Batalla 5 de mayo esq. Fuerte de Loreto, Col. Ejército de Oriente, C.P. 09230 Mexico City, Mexico
5
School of Higher Education of Agricultural Science, Autonomous University of Chiapas, Junction Costera Higway and Pueblo de Huehuetán, Huehuetán, C.P. 30660 Chiapas, Mexico
*
Author to whom correspondence should be addressed.
Received: 19 May 2017 / Revised: 13 July 2017 / Accepted: 14 July 2017 / Published: 25 July 2017
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Abstract

The Sechium edule Perla Negra cultivar is a recently-obtained biological material whose progenitors are S. edule var. nigrum minor and S. edule var. amarus silvestrys, the latter of which has been reported to have antiproliferative activity against the HeLa P-388 and L-929 cancer cell lines. The present study aimed to determine if the methanolic extract of the fruit of the Perla Negra cultivar had the same biological activity. The methanolic extract was phytochemically characterized by thin layer chromatography (TLC) and column chromatography (CC), identifying the terpenes and flavonoids. The compounds identified via high performance liquid chromatography (HPLC) were Cucurbitacins B, D, E, and I for the terpene fractions, and Rutin, Phlorizidin, Myricetin, Quercetin, Naringenin, Phloretin, Apigenin, and Galangin for the flavonoid fractions). Biological activity was evaluated with different concentrations of the methanolic extract in the HeLa cell line and normal lymphocytes. The methanolic extract inhibited the proliferation of HeLa cells (IC50 1.85 µg·mL−1), but the lymphocytes were affected by the extract (IC50 30.04 µg·mL−1). Some fractions, and the pool of all of them, showed inhibition higher than 80% at a concentration of 2.11 µg·mL−1. Therefore, the biological effect shown by the methanolic extract of the Perla Negra has some specificity in inhibiting tumor cells and not normal cells; an unusual feature among molecules investigated as potential biomedical agents. View Full-Text
Keywords: cancer; Perla Negra; chayote; biological activity cancer; Perla Negra; chayote; biological activity
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Salazar-Aguilar, S.; Ruiz-Posadas, L.M.; Cadena-Iñiguez, J.; Soto-Hernández, M.; Santiago-Osorio, E.; Aguiñiga-Sánchez, I.; Rivera-Martínez, A.R.; Aguirre-Medina, J.F. Sechium edule (Jacq.) Swartz, a New Cultivar with Antiproliferative Potential in a Human Cervical Cancer HeLa Cell Line. Nutrients 2017, 9, 798.

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