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Nutrients 2017, 9(6), 554; doi:10.3390/nu9060554

Annual Wormwood Leaf Inhibits the Adipogenesis of 3T3-L1 and Obesity in High-Fat Diet-Induced Obese Rats

1
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea
2
Department of Foods and Nutrition, Gyeongsang National University, Jinju 660-701, Korea
3
Department of Food Science and Biotechnology, Daegu University, Gyungsan 712-714, Korea
4
Department of Environmental Health Science, New York Medical College, Valhalla, NY 10595, USA
5
Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea
*
Author to whom correspondence should be addressed.
Received: 14 April 2017 / Revised: 16 May 2017 / Accepted: 23 May 2017 / Published: 28 May 2017
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Abstract

Annual wormwood (AW) (Artemisia annua L.) has anti-malarial, anti-bacterial, anti-oxidant, anti-tumour, and anti-inflammatory activities. In the present study, we evaluated the effects of annual wormwood leaves (AWL) on adipocyte differentiation in 3T3-L1 cells and high-fat diet (HFD)-induced obese rats. 3T3-L1 adipocytes and HFD-induced obese rats were treated with AWL, and its effect on gene expression was analyzed using RT-PCR and Western blotting experiments. Treatment with AWL effectively prevented triglyceride accumulation during adipogenesis in a dose-dependent manner. Consistently, AWL suppressed the differentiation of 3T3-L1 preadipocytes into adipocytes through the downregulation of dexamethasone, 3-isobutyl-1- methylxanthine, and insulin (DMI)-induced serine/threonine kinase protein kinase B (PKB/Akt) activation and the expression of adipogenic genes, including the CCAAT/enhancer binding protein-α (C/EBPα) and peroximal proliferator-activated receptor-γ (PPARγ). Moreover, the expression of adipocyte fatty acid-binding protein 4 (aP2), which is a known PPARγ-target gene, was downregulated by AWL treatment. Oral administration of AWL extracts significantly decreased the body weight gain, adipose tissue mass, adipocyte cell size, serum triglyceride (TG), and total cholesterol (TC) levels in HFD-induced obese rats. These results provide novel insight into the molecular mechanisms underlying the anti-obesity effects of AWL that are mediated by the downregulation of the expression of major adipogenic transcription factors, C/EBPα and PPARγ and Akt signalling. View Full-Text
Keywords: annual wormwood leaf; 3T3-L1 cells; adipogenesis; high-fat diet; PPARγ; Akt annual wormwood leaf; 3T3-L1 cells; adipogenesis; high-fat diet; PPARγ; Akt
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MDPI and ACS Style

Song, Y.; Lee, S.-J.; Jang, S.-H.; Kim, T.H.; Kim, H.-D.; Kim, S.-W.; Won, C.-K.; Cho, J.-H. Annual Wormwood Leaf Inhibits the Adipogenesis of 3T3-L1 and Obesity in High-Fat Diet-Induced Obese Rats. Nutrients 2017, 9, 554.

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