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Nutrients 2017, 9(5), 530; doi:10.3390/nu9050530

(−)-Epiafzelechin Protects against Ovariectomy-induced Bone Loss in Adult Mice and Modulate Osteoblastic and Osteoclastic Functions In Vitro

1,2,†
,
1,2,†
,
2,3
,
2
,
2,4
and
1,2,3,*
1
State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen 518057, China
2
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China
3
Shenzhen Key Laboratory of Food Biological Safety Control, Shenzhen 518057, China
4
Department of Chemistry, McGill University, Montréal, QC H3A 0B8, Canada
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 13 April 2017 / Revised: 8 May 2017 / Accepted: 11 May 2017 / Published: 22 May 2017
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Abstract

The present study was designed to characterize the bone protective effects of (−)-epiafzelechin (EAF), a flavan-3-ol, in mature ovariectomized mice model and its ability to stimulate osteoblastic activity and inhibit osteoclastic activity. Mature C57BL/6 mice (three to four months old) were either ovariectomised (OVX) or sham-operated and subjected to treatment (vehicle, 17β-oestradiol (E2, 200 μg/kg/day) or EAF (500 μg/kg/day) orally for six weeks. EAF and E2 significantly reduced urinary calcium (Ca) excretion, serum osteocalcin (OCN), and urinary deoxy-pyridinoline (DPD); increased bone mineral density (BMD); and improved micro-architectural properties in OVX mice. EAF significantly increased cell viability, alkaline phosphatise (ALP) activity, and collagen content, as well as runt-related transcriptional factor 2 (Runx2) mRNA expression in murine osteoblastic MC3T3-E1 cells. In addition, EAF significantly reduced the viability of osteoclast precursor murine leukemia monocyte RAW 264.7 cells and tartrate-resistant acid phosphatase (TRAP) activities in mature osteoclastic RAW 264.7 cells. EAF is a bioactive flavan-3-ol that protects estrogen deficiency-induced bone loss in OVX mice and exerts direct modulating effects in bone cells in vitro. View Full-Text
Keywords: (−)-epiafzelechin; flavan-3-ol; ovariectomised; osteogenesis; osteoclastogenesis; trabecular bone (−)-epiafzelechin; flavan-3-ol; ovariectomised; osteogenesis; osteoclastogenesis; trabecular bone
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Wong, K.-C.; Cao, S.; Dong, X.; Law, M.-C.; Chan, T.-H.; Wong, M.-S. (−)-Epiafzelechin Protects against Ovariectomy-induced Bone Loss in Adult Mice and Modulate Osteoblastic and Osteoclastic Functions In Vitro. Nutrients 2017, 9, 530.

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