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Nutrients 2017, 9(4), 332; doi:10.3390/nu9040332

Metabolic Impact of Light Phase-Restricted Fructose Consumption Is Linked to Changes in Hypothalamic AMPK Phosphorylation and Melatonin Production in Rats

1
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, #105 Alexander Fleming St., Campinas SP 13092-140, Brazil
2
Laboratory of Cell Signaling, Faculty of Medical Sciences, State University of Campinas, Carl von Linnaeus St., Campinas SP 13083-864, Brazil
3
Faculty of Applied Sciences, State University of Campinas, #1300 Pedro Zaccaria St., Limeira SP 13484-350, Brazil
4
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo SP 05508-900, Brazil
*
Author to whom correspondence should be addressed.
Received: 15 February 2017 / Revised: 12 March 2017 / Accepted: 16 March 2017 / Published: 27 March 2017
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Abstract

Recent studies show that the metabolic effects of fructose may vary depending on the phase of its consumption along with the light/dark cycle. Here, we investigated the metabolic outcomes of fructose consumption by rats during either the light (LPF) or the dark (DPF) phases of the light/dark cycle. This experimental approach was combined with other interventions, including restriction of chow availability to the dark phase, melatonin administration or intracerebroventricular inhibition of adenosine monophosphate-activated protein kinase (AMPK) with Compound C. LPF, but not DPF rats, exhibited increased hypothalamic AMPK phosphorylation, glucose intolerance, reduced urinary 6-sulfatoxymelatonin (6-S-Mel) (a metabolite of melatonin) and increased corticosterone levels. LPF, but not DPF rats, also exhibited increased chow ingestion during the light phase. The mentioned changes were blunted by Compound C. LPF rats subjected to dark phase-restricted feeding still exhibited increased hypothalamic AMPK phosphorylation but failed to develop the endocrine and metabolic changes. Moreover, melatonin administration to LPF rats reduced corticosterone and prevented glucose intolerance. Altogether, the present data suggests that consumption of fructose during the light phase results in out-of-phase feeding due to increased hypothalamic AMPK phosphorylation. This shift in spontaneous chow ingestion is responsible for the reduction of 6-S-Mel and glucose intolerance. View Full-Text
Keywords: fructose; out-of-phase feeding; AMPK; corticosterone; melatonin fructose; out-of-phase feeding; AMPK; corticosterone; melatonin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Faria, J.A.; de Araújo, T.M.F.; Razolli, D.S.; Ignácio-Souza, L.M.; Souza, D.N.; Bordin, S.; Anhê, G.F. Metabolic Impact of Light Phase-Restricted Fructose Consumption Is Linked to Changes in Hypothalamic AMPK Phosphorylation and Melatonin Production in Rats. Nutrients 2017, 9, 332.

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