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Nutrients 2017, 9(10), 1100; doi:10.3390/nu9101100

Balanced Diet-Fed Fat-1 Transgenic Mice Exhibit Lower Hindlimb Suspension-Induced Soleus Muscle Atrophy

1
Department of Internal Medicine, Faculty of Medicine, Universidad de La Frontera, Temuco 4780000, Chile
2
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil
3
School of Health Sciences, College of Health, Massey University, Auckland 0632, New Zealand
4
Laboratory of Lipids, Department of Food Science and Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, Brazil
5
Laboratory for Lipid Medicine and Technology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
6
Interdisciplinary Post-Graduate Program in Health Sciences, Cruzeiro do Sul University, São Paulo 05508-000, Brazil
*
Author to whom correspondence should be addressed.
Received: 18 August 2017 / Revised: 16 September 2017 / Accepted: 22 September 2017 / Published: 6 October 2017
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Abstract

The consequences of two-week hindlimb suspension (HS) on skeletal muscle atrophy were investigated in balanced diet-fed Fat-1 transgenic and C57BL/6 wild-type mice. Body composition and gastrocnemius fatty acid composition were measured. Skeletal muscle force, cross-sectional area (CSA), and signaling pathways associated with protein synthesis (protein kinase B, Akt; ribosomal protein S6, S6, eukaryotic translation initiation factor 4E-binding protein 1, 4EBP1; glycogen synthase kinase3-beta, GSK3-beta; and extracellular-signal-regulated kinases 1/2, ERK 1/2) and protein degradation (atrophy gene-1/muscle atrophy F-box, atrogin-1/MAFbx and muscle RING finger 1, MuRF1) were evaluated in the soleus muscle. HS decreased soleus muscle wet and dry weights (by 43% and 26%, respectively), muscle isotonic and tetanic force (by 29% and 18%, respectively), CSA of the soleus muscle (by 36%), and soleus muscle fibers (by 45%). Fat-1 transgenic mice had a decrease in the ω-6/ω-3 polyunsaturated fatty acids (PUFAs) ratio as compared with C57BL/6 wild-type mice (56%, p < 0.001). Fat-1 mice had lower soleus muscle dry mass loss (by 10%) and preserved absolute isotonic force (by 17%) and CSA of the soleus muscle (by 28%) after HS as compared with C57BL/6 wild-type mice. p-GSK3B/GSK3B ratio was increased (by 70%) and MuRF-1 content decreased (by 50%) in the soleus muscle of Fat-1 mice after HS. Balanced diet-fed Fat-1 mice are able to preserve in part the soleus muscle mass, absolute isotonic force and CSA of the soleus muscle in a disuse condition. View Full-Text
Keywords: Fat-1 mice; muscle disuse atrophy; ω-3 PUFAs; hindlimb suspension; protein synthesis/degradation signaling Fat-1 mice; muscle disuse atrophy; ω-3 PUFAs; hindlimb suspension; protein synthesis/degradation signaling
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MDPI and ACS Style

Marzuca-Nassr, G.N.; Murata, G.M.; Martins, A.R.; Vitzel, K.F.; Crisma, A.R.; Torres, R.P.; Mancini-Filho, J.; Kang, J.X.; Curi, R. Balanced Diet-Fed Fat-1 Transgenic Mice Exhibit Lower Hindlimb Suspension-Induced Soleus Muscle Atrophy. Nutrients 2017, 9, 1100.

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