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Nutrients 2016, 8(5), 267; doi:10.3390/nu8050267

Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium

1
Food Nutrition & Health Team, Food & Bio-based Products Group, AgResearch Grasslands Research Centre, Tennent Drive, Private Bag 11008, Palmerston North 4442, New Zealand
2
Riddet Institute, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand
3
AgResearch Grasslands, Palmerston North 4442, New Zealand
4
Food Assurance & Meat Quality Team, Food & Bio-based Products Group, Hopkirk Institute, Palmerston North 4442, New Zealand
*
Author to whom correspondence should be addressed.
Received: 9 February 2016 / Revised: 24 April 2016 / Accepted: 29 April 2016 / Published: 6 May 2016
(This article belongs to the Special Issue Dairy Products and Human Health)
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Abstract

Caprine milk contains the highest amount of oligosaccharides among domestic animals, which are structurally similar to human milk oligosaccharides (HMOs). This suggests caprine milk oligosaccharides may offer similar protective and developmental effects to that of HMOs. However, to date, studies using oligosaccharides from caprine milk have been limited. Thus, this study aimed to examine the impact of a caprine milk oligosaccharide-enriched fraction (CMOF) on barrier function of epithelial cell co-cultures of absorptive enterocytes (Caco-2 cells) and mucus-secreting goblet cells (HT29-MTX cells), that more closely simulate the cell proportions found in the small (90:10) and large intestine (75:25). Treatment of epithelial co-cultures with 0.4, 1.0, 2.0 and 4.0 mg/mL of CMOF was shown to have no effect on metabolic activity but did enhance cell epithelial barrier integrity as measured by trans-epithelial electrical resistance (TEER), in a dose-dependent manner. The CMOF at the maximum concentration tested (4.0 mg/mL) enhanced TEER, mucin gene expression and mucin protein abundance of epithelial co-cultures, all of which are essential components of intestinal barrier function. View Full-Text
Keywords: caprine milk oligosaccharides; Caco-2:HT29-MTX co-cultures; intestinal barrier function; mucin caprine milk oligosaccharides; Caco-2:HT29-MTX co-cultures; intestinal barrier function; mucin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Barnett, A.M.; Roy, N.C.; McNabb, W.C.; Cookson, A.L. Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium. Nutrients 2016, 8, 267.

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