Next Article in Journal
Dietary Natural Products for Prevention and Treatment of Liver Cancer
Previous Article in Journal
Fruit Intake and Abdominal Aortic Calcification in Elderly Women: A Prospective Cohort Study
Article Menu

Export Article

Open AccessArticle
Nutrients 2016, 8(3), 155; doi:10.3390/nu8030155

Lactobacillus fermentum Suo Attenuates HCl/Ethanol Induced Gastric Injury in Mice through Its Antioxidant Effects

1,2,†
,
3,4,5,†
,
3,4,5,†
,
3,4,5
,
3,4,5
,
6,* and 1,*
1
Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China
2
College of Food Science, Southwest University, Chongqing 400715, China
3
Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
4
Chongqing Collaborative Innovation Center of Functional Food, Chongqing University of Education, Chongqing 400067, China
5
Chongqing Engineering Technology Research Center for Functional Food, Chongqing University of Education, Chongqing 400067, China
6
Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 24 December 2015 / Revised: 24 February 2016 / Accepted: 1 March 2016 / Published: 10 March 2016
View Full-Text   |   Download PDF [2588 KB, uploaded 10 March 2016]   |  

Abstract

The purpose of the study was to determine the inhibitory effects of Lactobacillus fermentum Suo (LF-Suo) on HCl/ethanol induced gastric injury in ICR (Institute for Cancer Research) mice and explain the mechanism of these effects through the molecular biology activities of LF-Suo. The studied mice were divided into four groups: healthy, injured, LF-Suo-L and LF-Suo-H group. After the LF-Suo intragastric administration, the gastric injury area was reduced compared to the injured group. The serum MOT (motilin), SP (substance P), ET (endothelin) levels of LF-Suo treated mice were lower, and SS (somatostatin), VIP (vasoactive intestinal peptide) levels were higher than the injured group mice. The cytokine IL-6 (interleukin 6), IL-12 (interleukin 12), TNF-α (tumor necrosis factor-α) and IFN-γ (interferon-γ) serum levels were decreased after the LF-Suo treatment. The gastric tissues SOD (superoxide dismutase), GSH-Px (glutathione peroxidase), NO (nitric oxide) and activities of LF-Suo treated mice were increased and MDA (malondialdehyde) activity was decreased compared to the injured group mice. By the RT-PCR assay, LF-Suo raised the occludin, EGF (epidermal growth factor), EGFR (epidermal growth factor receptor), VEGF (vascular endothelial growth factor), Fit-1 (fms-like tyrosine kinase-1), IκB-α (inhibitor kappaB-α), nNOS (neuronal nitric oxide synthase), eNOS (endothelial nitric oxide synthase), Mn-SOD, Cu/Zn-SOD, CAT (catalase) mRNA or protein expressions and reduced the COX-2, NF-κB (nuclear factor kappaB), and iNOS (inducible nitric oxide synthase) expressions in gastric tissues compared to the gastric injured group mice. A high concentration (1.0 × 109 CFU/kg b.w.) of LF-Suo treatment showed stronger anti-gastric injury effects compared to a low concentration of (0.5 × 109 CFU/kg b.w.) of LF-Suo treatment. LF-Suo also showed strong survival in pH 3.0 man-made gastric juice and hydrophobic properties. These results indicate that LF-Suo has potential use as probiotics for its gastric injury treatment effects. View Full-Text
Keywords: Lactobacillus fermentum Suo; gastric injury; ethanol; antioxidant; mice Lactobacillus fermentum Suo; gastric injury; ethanol; antioxidant; mice
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Suo, H.; Zhao, X.; Qian, Y.; Sun, P.; Zhu, K.; Li, J.; Sun, B. Lactobacillus fermentum Suo Attenuates HCl/Ethanol Induced Gastric Injury in Mice through Its Antioxidant Effects. Nutrients 2016, 8, 155.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top