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Nutrients 2016, 8(10), 616; doi:10.3390/nu8100616

Effects of Phytoestrogen Extracts Isolated from Elder Flower on Hormone Production and Receptor Expression of Trophoblast Tumor Cells JEG-3 and BeWo, as well as MCF7 Breast Cancer Cells

1
Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University of Munich, Munich 80337, Germany
2
Department of Obstetrics and Gynaecology, University of Rostock, Rostock 18059, Germany
3
Department of Biological Sciences, University of Rostock, Rostock 18059, Germany
*
Author to whom correspondence should be addressed.
Received: 15 July 2016 / Accepted: 22 September 2016 / Published: 8 October 2016
(This article belongs to the Special Issue Polyphenols for Cancer Treatment or Prevention)
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Abstract

Hereinwe investigated the effect of elderflower extracts (EFE) and of enterolactone/enterodiol on hormone production and proliferation of trophoblast tumor cell lines JEG-3 and BeWo, as well as MCF7 breast cancer cells. The EFE was analyzed by mass spectrometry. Cells were incubated with various concentrations of EFE. Untreated cells served as controls. Supernatants were tested for estradiol production with an ELISA method. Furthermore, the effect of the EFE on ERα/ERβ/PR expression was assessed by immunocytochemistry. EFE contains a substantial amount of lignans. Estradiol production was inhibited in all cells in a concentration-dependent manner. EFE upregulated ERα in JEG-3 cell lines. In MCF7 cells, a significant ERα downregulation and PR upregulation were observed. The control substances enterolactone and enterodiol in contrast inhibited the expression of both ER and of PR in MCF7 cells. In addition, the production of estradiol was upregulated in BeWo and MCF7 cells in a concentration dependent manner. The downregulating effect of EFE on ERα expression and the upregulation of the PR expression in MFC-7 cells are promising results. Therefore, additional unknown substances might be responsible for ERα downregulation and PR upregulation. These findings suggest potential use of EFE in breast cancer prevention and/or treatment and warrant further investigation. View Full-Text
Keywords: lignans; isoflavones; elder flower; breast cancer; trophoblast tumor lignans; isoflavones; elder flower; breast cancer; trophoblast tumor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Schröder, L.; Richter, D.U.; Piechulla, B.; Chrobak, M.; Kuhn, C.; Schulze, S.; Abarzua, S.; Jeschke, U.; Weissenbacher, T. Effects of Phytoestrogen Extracts Isolated from Elder Flower on Hormone Production and Receptor Expression of Trophoblast Tumor Cells JEG-3 and BeWo, as well as MCF7 Breast Cancer Cells. Nutrients 2016, 8, 616.

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