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Nutrients 2015, 7(3), 1978-1991; doi:10.3390/nu7031978

Allomyrina dichotoma (Arthropoda: Insecta) Larvae Confer Resistance to Obesity in Mice Fed a High-Fat Diet

1
Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju-gun 565-851, Korea
2
Department of Bio-fibers and Materials Science, Kyungpook National University, Daegu 702-701, Korea
3
Department of Biochemistry, School of Medicine, Dongguk University, Gyeongju 780-714, Korea
*
Author to whom correspondence should be addressed.
Received: 5 January 2015 / Revised: 4 March 2015 / Accepted: 9 March 2015 / Published: 17 March 2015
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Abstract

To clarify the anti-obesity effect of Allomyrina dichotoma larvae (ADL), we previously reported that ADL block adipocyte differentiation on 3T3-L1 cell lines through downregulation of transcription factors, such as peroxisome proliferator-activated receptor-γ (PPARG) and CCAAT/enhancer binding protein-α (CEBPA). In this study, we tested whether ADL prevent obesity in mice fed a high-fat diet (HFD) and further investigated the mechanism underlying the effects of ADL. All mice were maintained on a normal-fat diet (NFD) for 1 week and then assigned to one of five treatment groups: (1) NFD; (2) HFD; (3) HFD and 100 mg·kg−1·day−1 ADL; (4) HFD and 3000 mg·kg−1·day−1ADL; or (5) HFD and 3000 mg·kg−1·day−1 yerba mate (Ilex paraguariensis, positive control). ADL and yerba mate were administered orally daily. Mice were fed experimental diets and body weight was monitored weekly for 6 weeks. Our results indicated that ADL reduced body weight gain, organ weight and adipose tissue volume in a dose-dependent manner. Body weight gain was approximately 22.4% lower compared to mice fed only HFD, but the difference did not reach the level of statistical significance. Real-time polymerase chain reaction (PCR) analysis revealed that gene expression levels of PPARG, CEBPA and lipoprotein lipase (LPL) in the epididymal fat tissue of HFD-fed mice receiving 3000 mg·kg−1·day−1 ADL were reduced by 12.4-, 25.7-, and 12.3-fold, respectively, compared to mice fed HFD only. Moreover, mice administered ADL had lower serum levels of triglycerides and leptin than HFD-fed mice that did not receive ADL. Taken together our results suggest that ADL and its constituent bioactive compounds hold potential for the treatment and prevention of obesity. View Full-Text
Keywords: high-fat diet (HFD); Allomyrina dichotoma; adipogenesis; lipogenesis; obesity high-fat diet (HFD); Allomyrina dichotoma; adipogenesis; lipogenesis; obesity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Yoon, Y.-I.; Chung, M.Y.; Hwang, J.-S.; Han, M.S.; Goo, T.-W.; Yun, E.-Y. Allomyrina dichotoma (Arthropoda: Insecta) Larvae Confer Resistance to Obesity in Mice Fed a High-Fat Diet. Nutrients 2015, 7, 1978-1991.

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