Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Nutrients 2013, 5(4), 1200-1217; doi:10.3390/nu5041200
Article

Vitamin C in Cultured Human (HeLa) Cells: Lack of Effect on DNA Protection and Repair

1,2,* , 2,3
, 2
, 2
 and 2
Received: 19 February 2013; in revised form: 18 March 2013 / Accepted: 20 March 2013 / Published: 9 April 2013
(This article belongs to the Special Issue Vitamin C and Human Health)
View Full-Text   |   Download PDF [1159 KB, updated 11 April 2013; original version uploaded 9 April 2013]
Abstract: Aims: Dietary antioxidants, including vitamin C, may be in part responsible for the cancer-preventive effects of fruits and vegetables. Human intervention trials with clinical endpoints have failed to confirm their protective effects, and mechanistic studies have given inconsistent results. Our aim was to investigate antioxidant/ pro-oxidant effects of vitamin C at the cellular level. Experimental approach: We have used the comet assay to investigate effects of vitamin C on DNA damage, antioxidant status, and DNA repair, in HeLa (human tumor) cells, and HPLC to measure uptake of vitamin C into cells. Results: Even at concentrations in the medium as high as 200 μM, vitamin C did not increase the background level of strand breaks or of oxidized purines in nuclear DNA. Vitamin C is taken up by HeLa cells and accumulates to mM levels. Preincubation of cells with vitamin C did not render them resistant to strand breakage induced by H2O2 or to purine oxidation by photosensitizer plus light. Vitamin C had no effect on the rate of repair of strand breaks or oxidized bases by HeLa cells. However, vitamin C at a concentration of less than 1 μM, or extract from cells preincubated for 6 h with vitamin C, was able to induce damage (strand breaks) in lysed, histone-depleted nuclei (nucleoids). Conclusion: In these cultured human cells, vitamin C displays neither antioxidant nor pro-oxidant properties; nor does it affect DNA strand break or base excision repair.
Keywords: DNA damage; DNA protection; DNA repair; vitamin C DNA damage; DNA protection; DNA repair; vitamin C
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Azqueta, A.; Costa, S.; Lorenzo, Y.; Bastani, N.E.; Collins, A.R. Vitamin C in Cultured Human (HeLa) Cells: Lack of Effect on DNA Protection and Repair. Nutrients 2013, 5, 1200-1217.

AMA Style

Azqueta A, Costa S, Lorenzo Y, Bastani NE, Collins AR. Vitamin C in Cultured Human (HeLa) Cells: Lack of Effect on DNA Protection and Repair. Nutrients. 2013; 5(4):1200-1217.

Chicago/Turabian Style

Azqueta, Amaya; Costa, Solange; Lorenzo, Yolanda; Bastani, Nasser E.; Collins, Andrew R. 2013. "Vitamin C in Cultured Human (HeLa) Cells: Lack of Effect on DNA Protection and Repair." Nutrients 5, no. 4: 1200-1217.


Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert