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Lectin Staining Shows no Evidence of Involvement of Glycocalyx/Mucous Layer Carbohydrate Structures in Development of Celiac Disease
Hans Christian Andersen Children's Hospital, Odense University Hospital, Sdr. Boulevard 29, Odense 5000, Denmark
Department of Clinical Immunology, Odense University Hospital, Sdr. Boulevard 29, Odense 5000, Denmark
Department of Pathology, Odense University Hospital, J.B. Winsløws Vej 15, 2., Odense 5000, Denmark
* Author to whom correspondence should be addressed.
Received: 23 September 2013; in revised form: 29 October 2013 / Accepted: 6 November 2013 / Published: 18 November 2013
Abstract: The presence of unique carbohydrate structures in the glycocalyx/mucous layer of the intestine may be involved in a susceptibility to celiac disease (CD) by serving as attachment sites for bacteria. This host-microbiota interaction may influence the development of CD and possibly other diseases with autoimmune components. We examined duodenal biopsies from a total of 30 children, of which 10 had both celiac disease (CD) and type 1 diabetes (T1D); 10 had CD alone; and 10 were suspected of having gastrointestinal disease, but had normal duodenal histology (non-CD controls). Patients with both CD and T1D were examined before and after remission following a gluten-free diet. We performed lectin histochemistry using peanut agglutinin (PNA) and Ulex europaeus agglutinin (UEA) staining for Gal-β(1,3)-GalNAc and Fucα1-2Gal-R, respectively, of the glycocalyx/mucous layer. The staining was scored based on dissemination of stained structures on a scale from 0 to 3. Evaluation of the scores revealed no difference between biopsies obtained before and after remission in the group of children with both CD and T1D. A comparison of this pre-remission group with the children who had CD alone or the non-CD controls also showed no significant differences. Based on our material, we found no indication that the presence of Gal-β(1,3)-GalNAc or Fucα1-2Gal-R is involved in the susceptibility to CD, or that the disease process affects the expression of these carbohydrates.
Keywords: pediatric celiac disease; type 1 diabetes; duodenal biopsies; glycosylation; lectin staining
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Toft-Hansen, H.; Nielsen, C.; Biagini, M.; Husby, S.; Lillevang, S.T. Lectin Staining Shows no Evidence of Involvement of Glycocalyx/Mucous Layer Carbohydrate Structures in Development of Celiac Disease. Nutrients 2013, 5, 4540-4552.
Toft-Hansen H, Nielsen C, Biagini M, Husby S, Lillevang ST. Lectin Staining Shows no Evidence of Involvement of Glycocalyx/Mucous Layer Carbohydrate Structures in Development of Celiac Disease. Nutrients. 2013; 5(11):4540-4552.
Toft-Hansen, Henrik; Nielsen, Christian; Biagini, Matteo; Husby, Steffen; Lillevang, Søren T. 2013. "Lectin Staining Shows no Evidence of Involvement of Glycocalyx/Mucous Layer Carbohydrate Structures in Development of Celiac Disease." Nutrients 5, no. 11: 4540-4552.