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Nutrients 2012, 4(2), 132-150; doi:10.3390/nu4020132
Article

Current Challenges in Detecting Food Allergens by Shotgun and Targeted Proteomic Approaches: A Case Study on Traces of Peanut Allergens in Baked Cookies

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Received: 25 December 2011; in revised form: 8 February 2012 / Accepted: 13 February 2012 / Published: 21 February 2012
(This article belongs to the Special Issue Foodomics 2011)
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Abstract: There is a need for selective and sensitive methods to detect the presence of food allergens at trace levels in highly processed food products. In this work, a combination of non-targeted and targeted proteomics approaches are used to illustrate the difficulties encountered in the detection of the major peanut allergens Ara h 1, Ara h 2 and Ara h 3 from a representative processed food matrix. Shotgun proteomics was employed for selection of the proteotypic peptides for targeted approaches via selective reaction monitoring. Peanut presence through detection of the proteotypic Ara h 3/4 peptides AHVQVVDSNGNR (m/z 432.5, 3+) and SPDIYNPQAGSLK (m/z 695.4, 2+) was confirmed and the developed method was able to detect peanut presence at trace levels (≥10 μg peanut g−1 matrix) in baked cookies.
Keywords: data dependent acquisition; peanut; food allergens; proteomics; shotgun; selective reaction monitoring; targeted data dependent acquisition; peanut; food allergens; proteomics; shotgun; selective reaction monitoring; targeted
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Pedreschi, R.; Nørgaard, J.; Maquet, A. Current Challenges in Detecting Food Allergens by Shotgun and Targeted Proteomic Approaches: A Case Study on Traces of Peanut Allergens in Baked Cookies. Nutrients 2012, 4, 132-150.

AMA Style

Pedreschi R, Nørgaard J, Maquet A. Current Challenges in Detecting Food Allergens by Shotgun and Targeted Proteomic Approaches: A Case Study on Traces of Peanut Allergens in Baked Cookies. Nutrients. 2012; 4(2):132-150.

Chicago/Turabian Style

Pedreschi, Romina; Nørgaard, Jørgen; Maquet, Alain. 2012. "Current Challenges in Detecting Food Allergens by Shotgun and Targeted Proteomic Approaches: A Case Study on Traces of Peanut Allergens in Baked Cookies." Nutrients 4, no. 2: 132-150.


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