Next Article in Journal / Special Issue
Current Challenges in Detecting Food Allergens by Shotgun and Targeted Proteomic Approaches: A Case Study on Traces of Peanut Allergens in Baked Cookies
Previous Article in Journal
Pro- and Synbiotics to Prevent Sepsis in Major Surgery and Severe Emergencies
Previous Article in Special Issue
Antiapoptotic and Antiautophagic Effects of Eicosapentaenoic Acid in Cardiac Myoblasts Exposed to Palmitic Acid
Article Menu

Export Article

Open AccessArticle
Nutrients 2012, 4(2), 112-131; doi:10.3390/nu4020112

Assessment of the Effect of High or Low Protein Diet on the Human Urine Metabolome as Measured by NMR

1
Department of Human Nutrition, Faculty of Life Sciences, University of Copenhagen, DK-1958 Frederiksberg, Denmark
2
Foss Analytical A/S, DK-3400 Hillerød, Denmark
3
Quality and Technology, Department of Food Science, Faculty of Life Sciences, University of Copenhagen, DK-1958 Frederiksberg, Denmark
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 30 January 2012 / Revised: 11 February 2012 / Accepted: 13 February 2012 / Published: 20 February 2012
(This article belongs to the Special Issue Foodomics 2011)
View Full-Text   |   Download PDF [4836 KB, uploaded 20 February 2012]   |  

Abstract

The objective of this study was to identify urinary metabolite profiles that discriminate between high and low intake of dietary protein during a dietary intervention. Seventy-seven overweight, non-diabetic subjects followed an 8-week low-calorie diet (LCD) and were then randomly assigned to a high (HP) or low (LP) protein diet for 6 months. Twenty-four hours urine samples were collected at baseline (prior to the 8-week LCD) and after dietary intervention; at months 1, 3 and 6, respectively. Metabolite profiling was performed by 1H NMR and chemometrics. Using partial least squares regression (PLS), it was possible to develop excellent prediction models for urinary nitrogen (root mean square error of cross validation (RMSECV) = 1.63 mmol/L; r = 0.89) and urinary creatinine (RMSECV = 0.66 mmol/L; r = 0.98). The obtained high correlations firmly establish the validity of the metabolomic approach since urinary nitrogen is a well established biomarker for daily protein consumption. The models showed that trimethylamine-N-oxide (TMAO) is correlated to urinary nitrogen. Furthermore, urinary creatine was found to be increased by the HP diet whereas citric acid was increased by the LP diet. Despite large variations in individual dietary intake, differentiated metabolite profiles were observed at the dietary group-level.
Keywords: metabolomics; metabolite profiles; dietary intervention; dietary protein; NMR spectroscopy; chemometrics metabolomics; metabolite profiles; dietary intervention; dietary protein; NMR spectroscopy; chemometrics
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Rasmussen, L.G.; Winning, H.; Savorani, F.; Toft, H.; Larsen, T.M.; Dragsted, L.O.; Astrup, A.; Engelsen, S.B. Assessment of the Effect of High or Low Protein Diet on the Human Urine Metabolome as Measured by NMR. Nutrients 2012, 4, 112-131.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top