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• Savini, M
• Cecchini, C
• Verdenelli, M
• Silvi, S
• Orpianesi, C
• Cresci, A
• [+] on Google Scholar
• Savini, M
• Cecchini, C
• Verdenelli, M
• Silvi, S
• Orpianesi, C
• Cresci, A
• [+] on PubMed
• Savini, M
• Cecchini, C
• Verdenelli, M
• Silvi, S
• Orpianesi, C
• Cresci, A

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Nutrients 2010, 2(3), 330-339; doi:10.3390/nu2030330

Article

Pilot-scale Production and Viability Analysis of Freeze-Dried Probiotic Bacteria Using Different Protective Agents

Michele Savini ¹ , Cinzia Cecchini ^1,* , Maria Cristina Verdenelli ² , Stefania Silvi ² , Carla Orpianesi ²  and Alberto Cresci ²

¹ Synbiotec S.r.l., Via d’Accorso 30/32, 62032 Camerino, Italy ² Dipartimento di Scienze Morfologiche e Biochimiche Comparate, Università di Camerino, Via Gentile III da Varano, 62032 Camerino, Italy

* Author to whom correspondence should be addressed.

Received: 23 December 2009; in revised form: 28 January 2010 / Accepted: 20 February 2010 / Published: 11 March 2010

(This article belongs to the Special Issue Food and Function 2009)

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Abstract: The functional food industry requires an improvement of probiotic strain stability during storage, especially when they are stored at room temperature. In this study, the viability of freeze-dried Lactobacillus rhamnosus IMC 501^® and Lactobacillus paracasei IMC 502^® using different protective agents (i.e., glycerine, mannitol, sorbitol, inulin, dextrin, Crystalean^®) was determined and compared with semi skimmed milk (SSM) control. No significant differences were observed between the tested protectants and the control (SSM) during storage at refrigerated conditions. During storage at room temperature, only glycerine was found to stabilize viability better than other tested substances.

Keywords: probiotics; prebiotics; cryoprotectans; freeze-drying; Lactobacillus rhamnosus; Lactobacillus paracasei

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