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Nutrients 2010, 2(3), 330-339; doi:10.3390/nu2030330
Article

Pilot-scale Production and Viability Analysis of Freeze-Dried Probiotic Bacteria Using Different Protective Agents

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Received: 23 December 2009 / Revised: 28 January 2010 / Accepted: 20 February 2010 / Published: 11 March 2010
(This article belongs to the Special Issue Food and Function 2009)
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Abstract

The functional food industry requires an improvement of probiotic strain stability during storage, especially when they are stored at room temperature. In this study, the viability of freeze-dried Lactobacillus rhamnosus IMC 501® and Lactobacillus paracasei IMC 502® using different protective agents (i.e., glycerine, mannitol, sorbitol, inulin, dextrin, Crystalean®) was determined and compared with semi skimmed milk (SSM) control. No significant differences were observed between the tested protectants and the control (SSM) during storage at refrigerated conditions. During storage at room temperature, only glycerine was found to stabilize viability better than other tested substances.
Keywords: probiotics; prebiotics; cryoprotectans; freeze-drying; Lactobacillus rhamnosus; Lactobacillus paracasei probiotics; prebiotics; cryoprotectans; freeze-drying; Lactobacillus rhamnosus; Lactobacillus paracasei
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Savini, M.; Cecchini, C.; Verdenelli, M.C.; Silvi, S.; Orpianesi, C.; Cresci, A. Pilot-scale Production and Viability Analysis of Freeze-Dried Probiotic Bacteria Using Different Protective Agents. Nutrients 2010, 2, 330-339.

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