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Pilot-scale Production and Viability Analysis of Freeze-Dried Probiotic Bacteria Using Different Protective Agents
Synbiotec S.r.l., Via d’Accorso 30/32, 62032 Camerino, Italy
Dipartimento di Scienze Morfologiche e Biochimiche Comparate, Università di Camerino, Via Gentile III da Varano, 62032 Camerino, Italy
* Author to whom correspondence should be addressed.
Received: 23 December 2009; in revised form: 28 January 2010 / Accepted: 20 February 2010 / Published: 11 March 2010
Abstract: The functional food industry requires an improvement of probiotic strain stability during storage, especially when they are stored at room temperature. In this study, the viability of freeze-dried Lactobacillus rhamnosus IMC 501® and Lactobacillus paracasei IMC 502® using different protective agents (i.e., glycerine, mannitol, sorbitol, inulin, dextrin, Crystalean®) was determined and compared with semi skimmed milk (SSM) control. No significant differences were observed between the tested protectants and the control (SSM) during storage at refrigerated conditions. During storage at room temperature, only glycerine was found to stabilize viability better than other tested substances.
Keywords: probiotics; prebiotics; cryoprotectans; freeze-drying; Lactobacillus rhamnosus; Lactobacillus paracasei
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MDPI and ACS Style
Savini, M.; Cecchini, C.; Verdenelli, M.C.; Silvi, S.; Orpianesi, C.; Cresci, A. Pilot-scale Production and Viability Analysis of Freeze-Dried Probiotic Bacteria Using Different Protective Agents. Nutrients 2010, 2, 330-339.
Savini M, Cecchini C, Verdenelli MC, Silvi S, Orpianesi C, Cresci A. Pilot-scale Production and Viability Analysis of Freeze-Dried Probiotic Bacteria Using Different Protective Agents. Nutrients. 2010; 2(3):330-339.
Savini, Michele; Cecchini, Cinzia; Verdenelli, Maria Cristina; Silvi, Stefania; Orpianesi, Carla; Cresci, Alberto. 2010. "Pilot-scale Production and Viability Analysis of Freeze-Dried Probiotic Bacteria Using Different Protective Agents." Nutrients 2, no. 3: 330-339.