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1H NMR Fingerprinting of Soybean Extracts, with Emphasis on Identification and Quantification of Isoflavones
Dipartimento di Chimica Organica e Industriale, Università di Parma, Via Usberti 17A, 43100-Parma, Italy
Dipartimento di Scienze Farmaceutiche, Università di Ferrara, Via Fossato di Mortara 17/19, 44100 Ferrara, Italy
* Author to whom correspondence should be addressed.
Received: 17 December 2009; Accepted: 24 February 2010 / Published: 26 February 2010
Abstract: 1H NMR spectra were recorded of methanolic extracts of seven soybean varieties (Glycine max.), cultivated using traditional and organic farming techniques. It was possible to identify signals belonging to the groups of amino acids, carbohydrates, organic acids and aromatic substances in the spectra. In the aromatic zone, the isoflavone signals were of particular interest: genistein, daidzein, genistin, daidzin, malonylgenistin, acetylgenistin, malonyldaidzin signals were assigned and these compounds were quantified, resulting in accordance with published data, and further demonstrating the potential of the NMR technique in food science.
Keywords: 1H NMR; soybean; isoflavones; fingerprinting
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Caligiani, A.; Palla, G.; Maietti, A.; Cirlini, M.; Brandolini, V. 1H NMR Fingerprinting of Soybean Extracts, with Emphasis on Identification and Quantification of Isoflavones. Nutrients 2010, 2, 280-289.
Caligiani A, Palla G, Maietti A, Cirlini M, Brandolini V. 1H NMR Fingerprinting of Soybean Extracts, with Emphasis on Identification and Quantification of Isoflavones. Nutrients. 2010; 2(3):280-289.
Caligiani, Augusta; Palla, Gerardo; Maietti, Annalisa; Cirlini, Martina; Brandolini, Vincenzo. 2010. "1H NMR Fingerprinting of Soybean Extracts, with Emphasis on Identification and Quantification of Isoflavones." Nutrients 2, no. 3: 280-289.