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Nutrients 2018, 10(2), 186; https://doi.org/10.3390/nu10020186

Modulation of miRNAs by Vitamin C in Human Bone Marrow Stromal Cells

1
Department of Pathology, Augusta University, Augusta, GA 30912, USA
2
Cellular Biology and Anatomy, Augusta University, Augusta, GA 30912, USA
3
Department of Orthopaedic Surgery, Augusta University, Augusta, GA 30912, USA
4
Institute of Regenerative and Reparative Medicine, Augusta University, Augusta, GA 30912, USA
*
Author to whom correspondence should be addressed.
Received: 22 November 2017 / Revised: 30 January 2018 / Accepted: 2 February 2018 / Published: 8 February 2018
(This article belongs to the Special Issue Nutrigenomics)
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Abstract

MicroRNAs (miRNAs) are small (18–25 nucleotides), noncoding RNAs that have been identified as potential regulators of bone marrow stromal cell (BMSC) proliferation, differentiation, and musculoskeletal development. Vitamin C is known to play a vital role in such types of biological processes through various different mechanisms by altering mRNA expression. We hypothesized that vitamin C mediates these biological processes partially through miRNA regulation. We performed global miRNA expression analysis on human BMSCs following vitamin C treatment using microarrays containing human precursor and mature miRNA probes. Bioinformatics analyses were performed on differentially expressed miRNAs to identify novel target genes and signaling pathways. Our bioinformatics analysis suggested that the miRNAs may regulate multiple stem cell-specific signaling pathways such as cell adhesion molecules (CAMs), fatty acid biosynthesis and hormone signaling pathways. Furthermore, our analysis predicted novel stem cell proliferation and differentiation gene targets. The findings of the present study demonstrate that vitamin C can have positive effects on BMSCs in part by regulating miRNA expression. View Full-Text
Keywords: bone marrow stromal cells; vitamin C; miRNA bone marrow stromal cells; vitamin C; miRNA
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Kolhe, R.; Mondal, A.K.; Pundkar, C.; Periyasamy-Thandavan, S.; Mendhe, B.; Hunter, M.; Isales, C.M.; Hill, W.D.; Hamrick, M.W.; Fulzele, S. Modulation of miRNAs by Vitamin C in Human Bone Marrow Stromal Cells. Nutrients 2018, 10, 186.

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