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Nutrients 2018, 10(1), 20; doi:10.3390/nu10010020

Leonurus japonicus Houtt Attenuates Nonalcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Cells and Mice Fed a High-Fat Diet

Korea Institute of Oriental Medicine, 70 Cheomdan-Ro, Dong-Gu, Daegu 41062, Korea
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Received: 3 November 2017 / Revised: 15 December 2017 / Accepted: 22 December 2017 / Published: 25 December 2017
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Abstract

We investigated the effects of a Leonurus japonicus ethanol extract (LJE) on nonalcoholic fatty liver disease (NAFLD). An in vitro model of hepatic steatosis was treated with 1 mM free fatty acid (FFA) in HepG2 cells. An in vivo NAFLD model was established using C57BL/6 mice fed a high-fat diet (HFD) and administered LJE (100 or 200 mg/kg) orally for 14 weeks. LJE treatment suppressed lipid accumulation and intracellular triglyceride levels significantly in a concentration-dependent manner in HepG2 cells. Moreover, LJE significantly reduced the expression of sterol regulatory element binding protein 1-c, and its downstream genes, which are associated with lipogenesis, in HepG2 cells. In HFD-fed mice, LJE treatment decreased body weight significantly and decreased serum alanine transaminase levels to normal values, concurrent with a decrease in hepatic lipid accumulation. Furthermore, LJE supplementation ameliorated insulin sensitivity by decreasing serum glucose and insulin levels. LJE improved hepatic steatosis by increasing the expression of phosphorylated AMP-activated protein kinase and peroxisome proliferator-activated receptor-α in HFD-fed mice and FFA-treated HepG2 cells. The results suggested that LJE might be a potential therapeutic agent to treat NAFLD. View Full-Text
Keywords: free fatty acids; HepG2 cells; high-fat diet; obesity; Leonurus japonicus; nonalcoholic fatty liver disease free fatty acids; HepG2 cells; high-fat diet; obesity; Leonurus japonicus; nonalcoholic fatty liver disease
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Lee, M.-R.; Park, K.I.; Ma, J.Y. Leonurus japonicus Houtt Attenuates Nonalcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Cells and Mice Fed a High-Fat Diet. Nutrients 2018, 10, 20.

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