Development and Optimization of a Fluorescent Differential Display PCR System for Analyzing the Stress Response in Lactobacillus sakei Strains

doi:10.3390/nu1020210

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Nutrients 2009, 1(2), 210-223; doi:10.3390/nu1020210

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Development and Optimization of a Fluorescent Differential Display PCR System for Analyzing the Stress Response in Lactobacillus sakei Strains

Maria Grazia Bonomo * , Maria Anna Sico , Simona Grieco and Giovanni Salzano

Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università degli Studi della Basilicata, Viale dell’Ateneo Lucano, 10, 85100 Potenza, Italy

* Author to whom correspondence should be addressed.

Received: 17 September 2009 / Accepted: 25 November 2009 / Published: 30 November 2009

(This article belongs to the Special Issue Foodomics 2009)

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Abstract: Lactobacillus sakei is widely used as starter in the production process of Italian fermented sausages and its growth and survival are affected by various factors. We studied the differential expression of genome in response to different stresses by the fluorescent differential display (FDD) technique. This study resulted in the development and optimization of an innovative technique, with a high level of reproducibility and quality, which allows the identification of gene expression changes associated with different microbial behaviours under different growth conditions.

Keywords: Fluorescent Differential Display PCR; Lactobacillus sakei; stress response

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MDPI and ACS Style

Bonomo, M.G.; Sico, M.A.; Grieco, S.; Salzano, G. Development and Optimization of a Fluorescent Differential Display PCR System for Analyzing the Stress Response in Lactobacillus sakei Strains. Nutrients 2009, 1, 210-223.

AMA Style

Bonomo M.G., Sico M.A., Grieco S., Salzano G. Development and Optimization of a Fluorescent Differential Display PCR System for Analyzing the Stress Response in Lactobacillus sakei Strains. Nutrients. 2009; 1(2):210-223.

Chicago/Turabian Style

Bonomo, Maria Grazia; Sico, Maria Anna; Grieco, Simona; Salzano, Giovanni. 2009. "Development and Optimization of a Fluorescent Differential Display PCR System for Analyzing the Stress Response in Lactobacillus sakei Strains." Nutrients 1, no. 2: 210-223.

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