Next Article in Journal / Special Issue
Development of Biodegradable Polycation-Based Inhalable Dry Gene Powders by Spray Freeze Drying
Previous Article in Journal / Special Issue
Image-Guided Hydrodynamic Gene Delivery: Current Status and Future Directions
Article Menu

Export Article

Open AccessCommunication
Pharmaceutics 2015, 7(3), 224-232; doi:10.3390/pharmaceutics7030224

Screening for Methylated Poly(⌊-histidine) with Various Dimethylimidazolium/Methylimidazole/Imidazole Contents as DNA Carrier

Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Keiji Itaka
Received: 30 May 2015 / Revised: 5 August 2015 / Accepted: 20 August 2015 / Published: 25 August 2015
(This article belongs to the Special Issue New Paradigm of Gene Therapy)
View Full-Text   |   Download PDF [957 KB, uploaded 25 August 2015]   |  

Abstract

Methylated poly(l-histidine) (PLH-Me), our original polypeptide, has controlled the contents of dimethylimidazolium, τ/π-methylimidazole and imidazole groups for efficient gene delivery. The screening for the PLH-Me as DNA carrier has been carried out by use of the PLH with 25 mol% (τ-methyl, 16 mol%; π-methyl, 17 mol%; deprotonated imidazole, 41 mol%), 68 mol% (τ-methyl, 16 mol%; π-methyl, 8 mol%; deprotonated imidazole, 8 mol%) and 87 mol% (τ-methyl, 7 mol%; π-methyl, 4 mol%; deprotonated imidazole, 2 mol%) dimethylimidazolium groups, that is, PLH-Me(25), PLH-Me(68) and PLH-Me(87), respectively. The screening of the chemical structure of PLH-Me has been carried out for DNA carrier properties, which are the stability of its DNA polyion complexes and gene expression. The DNA complexes with the 25 mol% and 68 mol% dimethylated PLH-Me possessed almost same ability to retain DNA, as compared with the 87 mol% dimethylated PLH-Me, which was examined by competitive exchange with dextran sulfate. From the gene transfection experiment against HepG2 cells, human hepatoma cell line, the PLH-Me(25)/DNA complex was revealed to mediate highest gene expression. These results suggest that the dimethyl-imidazolium/methylimidazole/imidazole balance of the PLH-Me is important for DNA carrier design. View Full-Text
Keywords: methylated poly(l-histidine); DNA carrier; dimethylimidazolium group; τ/π-methylimidazole group; imidazole group methylated poly(l-histidine); DNA carrier; dimethylimidazolium group; τ/π-methylimidazole group; imidazole group
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Asayama, S.; Kumagai, T.; Kawakami, H. Screening for Methylated Poly(⌊-histidine) with Various Dimethylimidazolium/Methylimidazole/Imidazole Contents as DNA Carrier. Pharmaceutics 2015, 7, 224-232.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Pharmaceutics EISSN 1999-4923 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top