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Pharmaceutics 2018, 10(3), 99; https://doi.org/10.3390/pharmaceutics10030099

Cellular Uptake Behaviors of Rigidity-Tunable Dendrimers

1
Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China
2
Chongqing Engineering Research Center for Micro-Nano Biomedical Materials and Devices, Chongqing 400715, China
*
Author to whom correspondence should be addressed.
Received: 19 June 2018 / Revised: 12 July 2018 / Accepted: 17 July 2018 / Published: 19 July 2018
(This article belongs to the Special Issue Dendrimers in Nanomedical Applications: Update and Future Directions)
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Abstract

Understanding of the interaction between cells and nanoparticles (NPs) is critical. Despite numerous attempts to understand the effect of several parameters of NPs on their cellular uptake behaviors, such as size, shape, surface chemistry, etc., limited information is available regarding NP rigidity. Herein, we investigate the effect of rigidity on cellular uptake behaviors of NPs, using generation 5 poly(amidoamine) dendrimer as a model. By harnessing the abundant inner cavity, their rigidity could be effectively regulated by forming size-tunable gold NPs. The NPs thus formed were well characterized and displayed similar hydrodynamic size, surface potential, fluorescence intensity, and distinct rigidity (owing to differences in the size of the Au core). Flow cytometry analysis revealed a positive correlation between NP rigidity and cellular uptake of NPs. Confocal microscopic evaluation revealed that the entrapped gold NPs may affect the intracellular localization of the internalized dendrimers. The present findings can potentially guide the preparation of suitable NPs for biomedical applications. View Full-Text
Keywords: PAMAM dendrimers; gold nanoparticles; rigidity; cellular uptake PAMAM dendrimers; gold nanoparticles; rigidity; cellular uptake
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Liu, H.; Wang, J.; Li, W.; Hu, J.; Wang, M.; Kang, Y. Cellular Uptake Behaviors of Rigidity-Tunable Dendrimers. Pharmaceutics 2018, 10, 99.

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