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Pharmaceutics 2018, 10(3), 84; https://doi.org/10.3390/pharmaceutics10030084

Development of a Column-Switching HPLC-MS/MS Method and Clinical Application for Determination of Ethyl Glucuronide in Hair in Conjunction with AUDIT for Detecting High-Risk Alcohol Consumption

1
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-gu, Daegu 42601, Korea
2
Bugok National Hospital, 145 Bugok-ro, Bugok-myeon, Changnyeong-gun, Gyeongsangnam-do 50365, Korea
These authors contributed equally.
*
Author to whom correspondence should be addressed.
Received: 17 May 2018 / Revised: 22 June 2018 / Accepted: 28 June 2018 / Published: 4 July 2018
(This article belongs to the Special Issue Drug Metabolism, Pharmacokinetics and Bioanalysis)
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Abstract

It is critical to assess the severity of alcohol consumption in certain diseases such as alcohol liver disease and alcohol addiction. Ethyl glucuronide (EtG) is a highly stable metabolite of ethanol in hair; thus, it was proposed as a long-term monitoring marker for alcohol consumption. Therefore, an HPLC-MS/MS method for EtG in hair was developed and applied to a clinical setting to assess the relevance of the EtG concentration and/or the Alcohol Use Disorders Identification Test (AUDIT) score to high-risk alcohol consumption. EtG was extracted from 10 mg of hair using water and analyzed using on-line sample purification coupled to HPLC-MS/MS. The diagnostic performances of the EtG concentration and/or the AUDIT score for detecting high-risk alcohol consumption were statistically evaluated between alcohol addicts (n = 44) and average alcohol users (n = 19). The on-line sample purification resulted in labor-saving with smaller sample amount. Both the EtG concentrations (4.0–587.4 pg/mg vs. 12.9–74.9 pg/mg) and the AUDIT scores (4–40 vs. 5–28) obtained from the alcohol addicts were significantly higher than those from the average alcohol users. The performance evaluation demonstrated that the integration score of the EtG concentration and the AUDIT score increased diagnostic performance for high-risk alcohol consumption. View Full-Text
Keywords: ethyl glucuronide; hair; HPLC-MS/MS; AUDIT score; alcohol addiction ethyl glucuronide; hair; HPLC-MS/MS; AUDIT score; alcohol addiction
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Kim, Y.G.; Hwang, J.; Choi, H.; Lee, S. Development of a Column-Switching HPLC-MS/MS Method and Clinical Application for Determination of Ethyl Glucuronide in Hair in Conjunction with AUDIT for Detecting High-Risk Alcohol Consumption. Pharmaceutics 2018, 10, 84.

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