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Viruses 2017, 9(8), 231; doi:10.3390/v9080231

Adaption of FMDV Asia-1 to Suspension Culture: Cell Resistance Is Overcome by Virus Capsid Alterations

1
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald–Insel Riems, Germany
2
Merck KGaA, Merck Life Sciences, Upstream R&D, Frankfurter Straße 250, 64293 Darmstadt, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Received: 2 June 2017 / Revised: 7 August 2017 / Accepted: 14 August 2017 / Published: 18 August 2017
(This article belongs to the Section Animal Viruses)
View Full-Text   |   Download PDF [2320 KB, uploaded 21 August 2017]   |  

Abstract

Foot-and-mouth disease virus (FMDV) causes a highly contagious disease with catastrophic economic impact for affected countries. BHK21 suspension cells are preferred for the industrial production of FMDV vaccine antigen, but not all virus strains can be successfully propagated in these cells. Serotype Asia-1 is often affected by this phenomenon. In this study, the Asia-1 strain Shamir was used to examine viral, cellular and environmental factors that contribute to resistance to cell culture infection. Cell media composition, pH and ammonium chloride concentration did not affect Asia-1 differently than other serotypes. Virus replication after transfection of viral genome was not impaired, but the adhesion to the cells was markedly reduced for Asia-1 in comparison to serotype A. The Asia-1 Shamir virus was successfully adapted to grow in the resistant cells by using a closely related but susceptible cell line. Sequence analysis of the adapted virus revealed two distinct mutations in the capsid protein VP1 that might mediate cell attachment and entry. View Full-Text
Keywords: foot-and-mouth disease virus; BHK21; susceptibility; resistance; serotype Asia-1; adaption; suspension cells; adhesion; receptor; antigen production foot-and-mouth disease virus; BHK21; susceptibility; resistance; serotype Asia-1; adaption; suspension cells; adhesion; receptor; antigen production
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Dill, V.; Hoffmann, B.; Zimmer, A.; Beer, M.; Eschbaumer, M. Adaption of FMDV Asia-1 to Suspension Culture: Cell Resistance Is Overcome by Virus Capsid Alterations. Viruses 2017, 9, 231.

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