Next Article in Journal
Telomerase Induction in HPV Infection and Oncogenesis
Previous Article in Journal
Development of Polioencephalomyelitis in Cesarean-Derived Colostrum-Deprived Pigs Following Experimental Inoculation with Either Teschovirus A Serotype 2 or Serotype 11
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Viruses 2017, 9(7), 178; doi:10.3390/v9070178

Almond Skin Extracts Abrogate HSV-1 Replication by Blocking Virus Binding to the Cell

Department of Chemical Biological Pharmaceutical and Environmental Sciences, University of Messina, Messina 98166, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Curt Hagedorn
Received: 7 June 2017 / Revised: 26 June 2017 / Accepted: 28 June 2017 / Published: 10 July 2017
(This article belongs to the Section Antivirals & Vaccines)
View Full-Text   |   Download PDF [3164 KB, uploaded 11 July 2017]   |  

Abstract

The aim of the present research was to determine the effect of almond skin extracts on herpes simplex virus 1 (HSV-1) replication. Drug-resistant strains of HSV frequently develop following therapeutic treatment. Therefore, the discovery of novel anti-HSV drugs deserves great effort. Here, we tested both natural (NS) and blanched (BS) polyphenols-rich almond skin extracts against HSV-1. HPLC analysis showed that the prevalent compounds in NS and BS extracts contributing to their antioxidant activity were quercetin, epicatechin and catechin. Results of cell viability indicated that NS and BS extracts were not toxic to cultured Vero cells. Furthermore, NS extracts were more potent inhibitors of HSV-1 than BS extracts, and this trend was in agreement with different concentrations of flavonoids. The plaque forming assay, Western blot and real-time PCR were used to demonstrate that NS extracts were able to block the production of infectious HSV-1 particles. In addition, the viral binding assay demonstrated that NS extracts inhibited HSV-1 adsorption to Vero cells. Our conclusion is that natural products from almond skin extracts are an extraordinary source of antiviral agents and provide a novel treatment against HSV-1 infections. View Full-Text
Keywords: herpes simplex virus 1; flavonoids; antiviral activity; binding mechanisms; almond skin herpes simplex virus 1; flavonoids; antiviral activity; binding mechanisms; almond skin
Figures

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Bisignano, C.; Mandalari, G.; Smeriglio, A.; Trombetta, D.; Pizzo, M.M.; Pennisi, R.; Sciortino, M.T. Almond Skin Extracts Abrogate HSV-1 Replication by Blocking Virus Binding to the Cell. Viruses 2017, 9, 178.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top