Next Article in Journal
Two Synechococcus genes, Two Different Effects on Cyanophage Infection
Previous Article in Journal
Characterization and Temperature Dependence of Arctic Micromonas polaris Viruses
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Viruses 2017, 9(6), 132; doi:10.3390/v9060132

Real-Time Expression Analysis of Selected Anticarsia gemmatalis multiple nucleopolyhedrovirus Gene Promoters during Infection of Permissive, Semipermissive and Nonpermissive Cell Lines

1
Laboratory of Baculovirus, Cell Biology Department, University of Brasília, 70910–900 Brasília-DF, Brazil
2
Laboratory of Insect Virology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, 97105–900 Santa Maria-RS, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Karyn Johnson
Received: 26 March 2017 / Revised: 22 May 2017 / Accepted: 24 May 2017 / Published: 1 June 2017
(This article belongs to the Section Insect Viruses)
View Full-Text   |   Download PDF [3335 KB, uploaded 23 June 2017]   |  

Abstract

Baculovirus infection follows a transcriptionally controlled sequence of gene expression that occurs by activation of different viral gene promoter sequences during infection. This sequence of promoter activation may be disrupted by cellular defenses against viral infection, which might interfere with viral progeny formation. In this work, the activity of the ie1, gp64, lef-1, vp39, p6.9 and polh promoters of the Anticarsia gemmatalis multiple nucleopolyhedrovirus was assessed during infection of permissive, semipermissive and nonpermissive cell lines by a novel methodology that detects reporter protein luminescence in real-time. This technique allowed us to characterize in rich detail the AgMNPV promoters in permissive cell lines and revealed differential profiles of expression in cells with limited permissivity that correlate well with limitations in viral DNA replication. Semipermissive and nonpermissive cell lines presented delays and restrictions in late and very late promoter expression. Cells undergoing apoptosis did not inhibit late gene expression; however, viral progeny formation is severely affected. This work demonstrates the application of the real-time luminescence detection methodology and how the promoter expression profile may be used to diagnose cellular permissivity to baculovirus infection. View Full-Text
Keywords: Baculovirus; Anticarsia gemmatalis; cell infection; permissive; nonpermissive; host range; promoter; hyperexpression; apoptosis Baculovirus; Anticarsia gemmatalis; cell infection; permissive; nonpermissive; host range; promoter; hyperexpression; apoptosis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Morgado, F.S.; Ardisson-Araújo, D.M.P.; Ribeiro, B.M. Real-Time Expression Analysis of Selected Anticarsia gemmatalis multiple nucleopolyhedrovirus Gene Promoters during Infection of Permissive, Semipermissive and Nonpermissive Cell Lines. Viruses 2017, 9, 132.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top