Diversity of dsDNA Viruses in a South African Hot Spring Assessed by Metagenomics and Microscopy
AbstractThe current view of virus diversity in terrestrial hot springs is limited to a few sampling sites. To expand our current understanding of hot spring viral community diversity, this study aimed to investigate the first African hot spring (Brandvlei hot spring; 60 °C, pH 5.7) by means of electron microscopy and sequencing of the virus fraction. Microscopy analysis revealed a mixture of regular- and ‘jumbo’-sized tailed morphotypes (Caudovirales), lemon-shaped virions (Fuselloviridae-like; salterprovirus-like) and pleiomorphic virus-like particles. Metavirome analysis corroborated the presence of His1-like viruses and has expanded the current clade of salterproviruses using a polymerase B gene phylogeny. The most represented viral contig was to a cyanophage genome fragment, which may underline basic ecosystem functioning provided by these viruses. Furthermore, a putative Gemmata-related phage was assembled with high coverage, a previously undocumented phage-host association. This study demonstrated that a moderately thermophilic spring environment contained a highly novel pool of viruses and should encourage future characterization of a wider temperature range of hot springs throughout the world. View Full-Text
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Zablocki, O.; van Zyl, L.J.; Kirby, B.; Trindade, M. Diversity of dsDNA Viruses in a South African Hot Spring Assessed by Metagenomics and Microscopy. Viruses 2017, 9, 348.
Zablocki O, van Zyl LJ, Kirby B, Trindade M. Diversity of dsDNA Viruses in a South African Hot Spring Assessed by Metagenomics and Microscopy. Viruses. 2017; 9(11):348.Chicago/Turabian Style
Zablocki, Olivier; van Zyl, Leonardo J.; Kirby, Bronwyn; Trindade, Marla. 2017. "Diversity of dsDNA Viruses in a South African Hot Spring Assessed by Metagenomics and Microscopy." Viruses 9, no. 11: 348.