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Viruses 2016, 8(9), 249; doi:10.3390/v8090249

Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft

1
Laboratory of Virology, Institute of Microbial Chemistry (BIKAKEN), 3-14-23 Kamiosaki, Shinagawa-ku, Tokyo 141-0021, Japan
2
Kitasato Institute for Life Sciences and Graduate School for Infection Control, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Received: 7 July 2016 / Revised: 1 September 2016 / Accepted: 2 September 2016 / Published: 10 September 2016
(This article belongs to the Section Animal Viruses)
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Abstract

The influenza glycoproteins, hemagglutinin (HA) and neuraminidase (NA), which are associated with the lipid raft, have the potential to initiate virion budding. However, the role of these viral proteins in infectious virion assembly is still unclear. In addition, it is not known how the viral ribonucleoprotein complex (vRNP) is tethered to the budding site. Here, we show that HA is necessary for the efficient progeny virion production and vRNP packaging in the virion. We also found that the level of HA does not affect the bundling of the eight vRNP segments, despite reduced virion production. Detergent solubilization and a subsequent membrane flotation analysis indicated that the accumulation of nucleoprotein, viral polymerases, NA, and matrix protein 1 (M1) in the lipid raft fraction was delayed without HA. Based on our results, we inferred that HA plays a role in the accumulation of viral components, including bundled vRNPs, at the lipid raft. View Full-Text
Keywords: influenza virus; lipid raft; virus budding; viral genome packaging; hemagglutinin influenza virus; lipid raft; virus budding; viral genome packaging; hemagglutinin
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MDPI and ACS Style

Takizawa, N.; Momose, F.; Morikawa, Y.; Nomoto, A. Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft. Viruses 2016, 8, 249.

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