Fluorescent and Bioluminescent Reporter Myxoviruses
AbstractThe advent of virus reverse genetics has enabled the incorporation of genetically encoded reporter proteins into replication-competent viruses. These reporters include fluorescent proteins which have intrinsic chromophores that absorb light and re-emit it at lower wavelengths, and bioluminescent proteins which are luciferase enzymes that react with substrates to produce visible light. The incorporation of these reporters into replication-competent viruses has revolutionized our understanding of molecular virology and aspects of viral tropism and transmission. Reporter viruses have also enabled the development of high-throughput assays to screen antiviral compounds and antibodies and to perform neutralization assays. However, there remain technical challenges with the design of replication-competent reporter viruses, and each reporter has unique advantages and disadvantages for specific applications. This review describes currently available reporters, design strategies for incorporating reporters into replication-competent paramyxoviruses and orthomyxoviruses, and the variety of applications for which these tools can be utilized both in vitro and in vivo. View Full-Text
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Rostad, C.A.; Currier, M.C.; Moore, M.L. Fluorescent and Bioluminescent Reporter Myxoviruses. Viruses 2016, 8, 214.
Rostad CA, Currier MC, Moore ML. Fluorescent and Bioluminescent Reporter Myxoviruses. Viruses. 2016; 8(8):214.Chicago/Turabian Style
Rostad, Christina A.; Currier, Michael C.; Moore, Martin L. 2016. "Fluorescent and Bioluminescent Reporter Myxoviruses." Viruses 8, no. 8: 214.
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