Next Article in Journal
In Vitro Synthesized RNA Generated from cDNA Clones of Both Genomic Components of Cucurbit yellow stunting disorder virus Replicates in Cucumber Protoplasts
Previous Article in Journal
Stress Beyond Translation: Poxviruses and More
Article Menu

Export Article

Open AccessArticle
Viruses 2016, 8(6), 168; doi:10.3390/v8060168

Ferrets as a Novel Animal Model for Studying Human Respiratory Syncytial Virus Infections in Immunocompetent and Immunocompromised Hosts

1
Viroclinics Biosciences, 3029 AK Rotterdam, The Netherlands
2
Department of Viroscience, Erasmus MC, 3015 CN Rotterdam, The Netherlands
3
Research Centre for Emerging Infections and Zoonoses, University of Veterinary Medicine, 30559 Hannover, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Received: 6 May 2016 / Revised: 3 June 2016 / Accepted: 5 June 2016 / Published: 14 June 2016
(This article belongs to the Section Animal Viruses)
View Full-Text   |   Download PDF [4757 KB, uploaded 14 June 2016]   |  

Abstract

Human respiratory syncytial virus (HRSV) is an important cause of severe respiratory tract disease in immunocompromised patients. Animal models are indispensable for evaluating novel intervention strategies in this complex patient population. To complement existing models in rodents and non-human primates, we have evaluated the potential benefits of an HRSV infection model in ferrets (Mustela putorius furo). Nine- to 12-month-old HRSV-seronegative immunocompetent or immunocompromised ferrets were infected with a low-passage wild-type strain of HRSV subgroup A (105 TCID50) administered by intra-tracheal or intra-nasal inoculation. Immune suppression was achieved by bi-daily oral administration of tacrolimus, mycophenolate mofetil, and prednisolone. Throat and nose swabs were collected daily and animals were euthanized four, seven, or 21 days post-infection (DPI). Virus loads were determined by quantitative virus culture and qPCR. We observed efficient HRSV replication in both the upper and lower respiratory tract. In immunocompromised ferrets, virus loads reached higher levels and showed delayed clearance as compared to those in immunocompetent animals. Histopathological evaluation of animals euthanized 4 DPI demonstrated that the virus replicated in the respiratory epithelial cells of the trachea, bronchi, and bronchioles. These animal models can contribute to an assessment of the efficacy and safety of novel HRSV intervention strategies. View Full-Text
Keywords: respiratory syncytial virus; animal models; ferret; viral loads; immunocompromised host respiratory syncytial virus; animal models; ferret; viral loads; immunocompromised host
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Stittelaar, K.J.; de Waal, L.; van Amerongen, G.; Veldhuis Kroeze, E.J.; Fraaij, P.L.; van Baalen, C.A.; van Kampen, J.J.; van der Vries, E.; Osterhaus, A.D.; de Swart, R.L. Ferrets as a Novel Animal Model for Studying Human Respiratory Syncytial Virus Infections in Immunocompetent and Immunocompromised Hosts. Viruses 2016, 8, 168.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top