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Viruses 2016, 8(5), 141; doi:10.3390/v8050141

A New Orbivirus Isolated from Mosquitoes in North-Western Australia Shows Antigenic and Genetic Similarity to Corriparta Virus but Does Not Replicate in Vertebrate Cells

1
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Australia
2
Public Health Virology Laboratory, Department of Health, Queensland Government, P.O. Box 594, Archerfield 4108, Australia
3
School of Pathology and Laboratory Medicine, The University of Western Australia, Nedlands 6009, Australia
4
Berrimah Veterinary Laboratory, Department of Primary Industries and Fisheries, Darwin 0828, Australia
These authors contributed equally to the work.
*
Author to whom correspondence should be addressed.
Academic Editor: Karyn Johnson
Received: 19 February 2016 / Revised: 27 April 2016 / Accepted: 10 May 2016 / Published: 20 May 2016
(This article belongs to the Section Insect Viruses)
View Full-Text   |   Download PDF [11293 KB, uploaded 20 May 2016]   |  

Abstract

The discovery and characterisation of new mosquito-borne viruses provides valuable information on the biodiversity of vector-borne viruses and important insights into their evolution. In this study, a broad-spectrum virus screening system, based on the detection of long double-stranded RNA in inoculated cell cultures, was used to investigate the presence of novel viruses in mosquito populations of northern Australia. We detected and isolated a new virus (tentatively named Parry’s Lagoon virus, PLV) from Culex annulirostris, Culex pullus, Mansonia uniformis and Aedes normanensis mosquitoes that shares genomic sequence similarities to Corriparta virus (CORV), a member of the Orbivirus genus of the family Reoviridae. Despite moderate to high (72.2% to 92.2%) amino acid identity across all proteins when compared to CORV, and demonstration of antigenic relatedness, PLV did not replicate in several vertebrate cell lines that were permissive to CORV. This striking phenotypic difference suggests that PLV has evolved to have a very restricted host range, indicative of a mosquito-only life cycle. View Full-Text
Keywords: insect-specific virus; reovirus; orbivirus; mosquito; arbovirus; virus discovery; Culex annulirostris; double-stranded RNA insect-specific virus; reovirus; orbivirus; mosquito; arbovirus; virus discovery; Culex annulirostris; double-stranded RNA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Harrison, J.J.; Warrilow, D.; McLean, B.J.; Watterson, D.; O’Brien, C.A.; Colmant, A.M.; Johansen, C.A.; Barnard, R.T.; Hall-Mendelin, S.; Davis, S.S.; Hall, R.A.; Hobson-Peters, J. A New Orbivirus Isolated from Mosquitoes in North-Western Australia Shows Antigenic and Genetic Similarity to Corriparta Virus but Does Not Replicate in Vertebrate Cells. Viruses 2016, 8, 141.

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