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Viruses 2016, 8(5), 136; doi:10.3390/v8050136

The Effect of MicroRNA bantam on Baculovirus AcMNPV Infection in Vitro and in Vivo

State Key Laboratory of Genetic Engineering, Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China
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Academic Editor: Johannes Jehle
Received: 3 March 2016 / Revised: 24 April 2016 / Accepted: 10 May 2016 / Published: 16 May 2016
(This article belongs to the Section Insect Viruses)
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Abstract

The role of microRNA bantam, one of the most abundant microRNAs in Sf9 cells, was studied for its role in baculovirus infection in vitro and in vivo. The expression level of bantam was increased after AcMNPV infection in Sf9 cells and in Spodoptera litura larvae. In Sf9 cells, application of bantam inhibitor or mimic altered the expression of many virus genes, the most affected gene being lef8, gp41 and p10, the expression level of which was increased by 8, 10 and 40 times, respectively, in the presence of bantam inhibitor. Virus DNA replication was decreased in the presence of bantam mimic and increased in the presence of bantam inhibitor in a dose dependent manner. However, the production of budded virus did not change significantly. Feeding the larvae of S. litura and Spodoptera exigua with bantam antagomiR, a more stable form of the inhibitor, resulted in an abnormal larval growth and a decreased pupation rate. In S. litura, larvae died 3.5 days sooner than the control when bantam antagomiR was applied, together with AcMNPV. In infected S. exigua, larval mortality increased from 47% without antagomiR to 80% with it. The results suggest that microRNA bantam plays an important role in insect growth, as well as in baculovirus-insect interaction. View Full-Text
Keywords: microRNA; bantam; baculovirus; virus-host interaction; biopesticide microRNA; bantam; baculovirus; virus-host interaction; biopesticide
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Shi, X.; Ran, Z.; Li, S.; Yin, J.; Zhong, J. The Effect of MicroRNA bantam on Baculovirus AcMNPV Infection in Vitro and in Vivo. Viruses 2016, 8, 136.

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