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Viruses 2016, 8(5), 125; doi:10.3390/v8050125

Engineering Hepadnaviruses as Reporter-Expressing Vectors: Recent Progress and Future Perspectives

Key Laboratory of Medical Molecular Virology (MOH & MOE) and Institutes of Biomedical Sciences, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China
These authors contributed equally to this work.
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Authors to whom correspondence should be addressed.
Academic Editor: Luis Martinez-Sobrido
Received: 29 February 2016 / Revised: 21 April 2016 / Accepted: 29 April 2016 / Published: 10 May 2016
(This article belongs to the Special Issue Replication-Competent Reporter-Expressing Viruses)
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Abstract

The Hepadnaviridae family of small, enveloped DNA viruses are characterized by a strict host range and hepatocyte tropism. The prototype hepatitis B virus (HBV) is a major human pathogen and constitutes a public health problem, especially in high-incidence areas. Reporter-expressing recombinant viruses are powerful tools in both studies of basic virology and development of antiviral therapeutics. In addition, the highly restricted tropism of HBV for human hepatocytes makes it an ideal tool for hepatocyte-targeting in vivo applications such as liver-specific gene delivery. However, compact genome organization and complex replication mechanisms of hepadnaviruses have made it difficult to engineer replication-competent recombinant viruses that express biologically-relevant cargo genes. This review analyzes difficulties associated with recombinant hepadnavirus vector development, summarizes and compares the progress made in this field both historically and recently, and discusses future perspectives regarding both vector design and application. View Full-Text
Keywords: recombinant virus; viral vector; gene delivery; HBV; DHBV recombinant virus; viral vector; gene delivery; HBV; DHBV
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Bai, W.; Cui, X.; Xie, Y.; Liu, J. Engineering Hepadnaviruses as Reporter-Expressing Vectors: Recent Progress and Future Perspectives. Viruses 2016, 8, 125.

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