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Viruses 2016, 8(5), 120; doi:10.3390/v8050120

Characterization of a Novel Polerovirus Infecting Maize in China

1
Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China
2
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
3
Key Laboratory of Pest Management of Horticultural Crop of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha 410125, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Thomas Hohn
Received: 22 March 2016 / Revised: 20 April 2016 / Accepted: 22 April 2016 / Published: 28 April 2016
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Abstract

A novel virus, tentatively named Maize Yellow Mosaic Virus (MaYMV), was identified from the field-grown maize plants showing yellow mosaic symptoms on the leaves collected from the Yunnan Province of China by the deep sequencing of small RNAs. The complete 5642 nucleotide (nt)-long genome of the MaYMV shared the highest nucleotide sequence identity (73%) to Maize Yellow Dwarf Virus-RMV. Sequence comparisons and phylogenetic analyses suggested that MaYMV represents a new member of the genus Polerovirus in the family Luteoviridae. Furthermore, the P0 protein encoded by MaYMV was demonstrated to inhibit both local and systemic RNA silencing by co-infiltration assays using transgenic Nicotiana benthamiana line 16c carrying the GFP reporter gene, which further supported the identification of a new polerovirus. The biologically-active cDNA clone of MaYMV was generated by inserting the full-length cDNA of MaYMV into the binary vector pCB301. RT-PCR and Northern blot analyses showed that this clone was systemically infectious upon agro-inoculation into N. benthamiana. Subsequently, 13 different isolates of MaYMV from field-grown maize plants in different geographical locations of Yunnan and Guizhou provinces of China were sequenced. Analyses of their molecular variation indicate that the 3′ half of P3–P5 read-through protein coding region was the most variable, whereas the coat protein- (CP-) and movement protein- (MP-)coding regions were the most conserved. View Full-Text
Keywords: Maize yellow mosaic virus; deep sequencing; infectious clone; suppressor; molecular variation; sRNA Maize yellow mosaic virus; deep sequencing; infectious clone; suppressor; molecular variation; sRNA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Chen, S.; Jiang, G.; Wu, J.; Liu, Y.; Qian, Y.; Zhou, X. Characterization of a Novel Polerovirus Infecting Maize in China. Viruses 2016, 8, 120.

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