Characterization of a Novel Polerovirus Infecting Maize in China
AbstractA novel virus, tentatively named Maize Yellow Mosaic Virus (MaYMV), was identified from the field-grown maize plants showing yellow mosaic symptoms on the leaves collected from the Yunnan Province of China by the deep sequencing of small RNAs. The complete 5642 nucleotide (nt)-long genome of the MaYMV shared the highest nucleotide sequence identity (73%) to Maize Yellow Dwarf Virus-RMV. Sequence comparisons and phylogenetic analyses suggested that MaYMV represents a new member of the genus Polerovirus in the family Luteoviridae. Furthermore, the P0 protein encoded by MaYMV was demonstrated to inhibit both local and systemic RNA silencing by co-infiltration assays using transgenic Nicotiana benthamiana line 16c carrying the GFP reporter gene, which further supported the identification of a new polerovirus. The biologically-active cDNA clone of MaYMV was generated by inserting the full-length cDNA of MaYMV into the binary vector pCB301. RT-PCR and Northern blot analyses showed that this clone was systemically infectious upon agro-inoculation into N. benthamiana. Subsequently, 13 different isolates of MaYMV from field-grown maize plants in different geographical locations of Yunnan and Guizhou provinces of China were sequenced. Analyses of their molecular variation indicate that the 3′ half of P3–P5 read-through protein coding region was the most variable, whereas the coat protein- (CP-) and movement protein- (MP-)coding regions were the most conserved. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 595 KB)
Share & Cite This Article
Chen, S.; Jiang, G.; Wu, J.; Liu, Y.; Qian, Y.; Zhou, X. Characterization of a Novel Polerovirus Infecting Maize in China. Viruses 2016, 8, 120.
Chen S, Jiang G, Wu J, Liu Y, Qian Y, Zhou X. Characterization of a Novel Polerovirus Infecting Maize in China. Viruses. 2016; 8(5):120.Chicago/Turabian Style
Chen, Sha; Jiang, Guangzhuang; Wu, Jianxiang; Liu, Yong; Qian, Yajuan; Zhou, Xueping. 2016. "Characterization of a Novel Polerovirus Infecting Maize in China." Viruses 8, no. 5: 120.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.