Next Article in Journal
Measles Virus Fusion Protein: Structure, Function and Inhibition
Previous Article in Journal
Constraints on the Genetic and Antigenic Variability of Measles Virus
Article Menu

Export Article

Open AccessArticle
Viruses 2016, 8(4), 115; doi:10.3390/v8040115

Intranasal Immunization with Influenza Virus-Like Particles Containing Membrane-Anchored Cholera Toxin B or Ricin Toxin B Enhances Adaptive Immune Responses and Protection against an Antigenically Distinct Virus

1
College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot 010018, China
2
Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China
3
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100730, China
4
Key Lab of Cellular and Molecular Immunology, Henan University School of Medicine, Kaifeng 475001, China
5
Jilin Medical University, Changchun 132013, China
6
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
7
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Nanjing 210009, China
8
School of Public Health, Shandong University, Jinan 250110, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Curt Hagedorn
Received: 8 January 2016 / Revised: 14 March 2016 / Accepted: 15 April 2016 / Published: 21 April 2016
(This article belongs to the Section Antivirals & Vaccines)
View Full-Text   |   Download PDF [2042 KB, uploaded 21 April 2016]   |  

Abstract

Vaccination is the most effective means to prevent influenza virus infection, although current approaches are associated with suboptimal efficacy. Here, we generated virus-like particles (VLPs) composed of the hemagglutinin (HA), neuraminidase (NA) and matrix protein (M1) of A/Changchun/01/2009 (H1N1) with or without either membrane-anchored cholera toxin B (CTB) or ricin toxin B (RTB) as molecular adjuvants. The intranasal immunization of mice with VLPs containing membrane-anchored CTB or RTB elicited stronger humoral and cellular immune responses when compared to mice immunized with VLPs alone. Administration of VLPs containing CTB or RTB significantly enhanced virus-specific systemic and mucosal antibody responses, hemagglutination inhibiting antibody titers, virus neutralizing antibody titers, and the frequency of virus-specific IFN-γ and IL-4 secreting splenocytes. VLPs with and without CTB or RTB conferred complete protection against lethal challenge with a mouse-adapted homologous virus. When challenged with an antigenically distinct H1N1 virus, all mice immunized with VLPs containing CTB or RTB survived whereas mice immunized with VLPs alone showed only partial protection (80% survival). Our results suggest that membrane-anchored CTB and RTB possess strong adjuvant properties when incorporated into an intranasally-delivered influenza VLP vaccine. Chimeric influenza VLPs containing CTB or RTB may represent promising vaccine candidates for improved immunological protection against homologous and antigenically distinct influenza viruses. View Full-Text
Keywords: influenza; virus-like particles; membrane-anchored cholera toxin B; ricin toxin B; cross-protection; intranasal administration influenza; virus-like particles; membrane-anchored cholera toxin B; ricin toxin B; cross-protection; intranasal administration
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ji, X.; Ren, Z.; Xu, N.; Meng, L.; Yu, Z.; Feng, N.; Sang, X.; Li, S.; Li, Y.; Wang, T.; Zhao, Y.; Wang, H.; Zheng, X.; Jin, H.; Li, N.; Yang, S.; Cao, J.; Liu, W.; Gao, Y.; Xia, X. Intranasal Immunization with Influenza Virus-Like Particles Containing Membrane-Anchored Cholera Toxin B or Ricin Toxin B Enhances Adaptive Immune Responses and Protection against an Antigenically Distinct Virus. Viruses 2016, 8, 115.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top