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Viruses 2016, 8(3), 68; doi:10.3390/v8030068

Cell-Free and Cell-Based Approaches to Explore the Roles of Host Membranes and Lipids in the Formation of Viral Replication Compartment Induced by Tombusviruses

Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
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Author to whom correspondence should be addressed.
Academic Editor: Andrew Tai
Received: 8 January 2016 / Revised: 18 February 2016 / Accepted: 22 February 2016 / Published: 3 March 2016
(This article belongs to the Special Issue Host Membranes and the Viral Infection Cycle)
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Abstract

Plant positive strand RNA viruses are intracellular infectious agents that take advantage of cellular lipids and membranes to support replication and protect viral RNA from degradation by host antiviral responses. In this review, we discuss how Tomato bushy stunt virus (TBSV) co-opts lipid transfer proteins and modulates lipid metabolism and transport to facilitate the assembly of the membrane-bound viral replicase complexes within intricate replication compartments. Identification and characterization of the proviral roles of specific lipids and proteins involved in lipid metabolism based on results from yeast (Saccharomyces cerevisiae) model host and cell-free approaches are discussed. The review also highlights the advantage of using liposomes with chemically defined composition to identify specific lipids required for TBSV replication. Remarkably, all the known steps in TBSV replication are dependent on cellular lipids and co-opted membranes. View Full-Text
Keywords: viral replicase complex; phospholipids; sterol; lipid metabolism; lipidomics; virus infection; yeast; in vitro replication; membrane contact site; lipid transfer proteins viral replicase complex; phospholipids; sterol; lipid metabolism; lipidomics; virus infection; yeast; in vitro replication; membrane contact site; lipid transfer proteins
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Nagy, P.D.; Pogany, J.; Xu, K. Cell-Free and Cell-Based Approaches to Explore the Roles of Host Membranes and Lipids in the Formation of Viral Replication Compartment Induced by Tombusviruses. Viruses 2016, 8, 68.

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