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Viruses 2015, 7(8), 4772-4782; doi:10.3390/v7082843

Artificial TALE as a Convenient Protein Platform for Engineering Broad-Spectrum Resistance to Begomoviruses

1
College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, Zhejiang, China
2
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, China
3
College of Agricultural and Food Science, Zhejiang Agricultural and Forestry University, Lin'an 311300, Zhejiang, China
4
Key Laboratory of Molecular Genetics, China National Tobacco Corporation, Guizhou, Institute of Tobacco Science, Guiyang 550083, Guizhou, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Thomas Hohn
Received: 10 May 2015 / Revised: 29 July 2015 / Accepted: 5 August 2015 / Published: 20 August 2015
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
View Full-Text   |   Download PDF [628 KB, uploaded 20 August 2015]   |  

Abstract

Transcription activator–like effectors (TALEs) are a class of sequence-specific DNA-binding proteins that utilize a simple and predictable modality to recognize target DNA. This unique characteristic allows for the rapid assembly of artificial TALEs, with high DNA binding specificity, to any target DNA sequences for the creation of customizable sequence-specific nucleases used in genome engineering. Here, we report the use of an artificial TALE protein as a convenient platform for designing broad-spectrum resistance to begomoviruses, one of the most destructive plant virus groups, which cause tremendous losses worldwide. We showed that artificial TALEs, which were assembled based on conserved sequence motifs within begomovirus genomes, could confer partial resistance in transgenic Nicotiana benthamiana to all three begomoviruses tested. Furthermore, the resistance was maintained even in the presence of their betasatellite. These results shed new light on the development of broad-spectrum resistance against DNA viruses, such as begomoviruses. View Full-Text
Keywords: Antivirus; Begomovirus; Broad-spectrum resistance; DNA-binding protein; Transcription activator–like effectors Antivirus; Begomovirus; Broad-spectrum resistance; DNA-binding protein; Transcription activator–like effectors
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Cheng, X.; Li, F.; Cai, J.; Chen, W.; Zhao, N.; Sun, Y.; Guo, Y.; Yang, X.; Wu, X. Artificial TALE as a Convenient Protein Platform for Engineering Broad-Spectrum Resistance to Begomoviruses. Viruses 2015, 7, 4772-4782.

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