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Viruses 2015, 7(8), 4169-4185; doi:10.3390/v7082815

BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape

1
College of Plant Science and Technology of Huazhong Agricultural University, Wuhan 430070, China
2
Xiaozhou Agricultural Service Center in Wanzhou District, Chongqing 404089, China
3
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Biologyand Genetic Breeding of Oil Crops, the Ministry of Agriculture, Wuhan 430062, China
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas Hohn
Received: 26 April 2015 / Revised: 7 July 2015 / Accepted: 17 July 2015 / Published: 27 July 2015
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Abstract

Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with different genus viruses by constructing BnSGS3-overexpressing (BnSGS3-Ov) and BnSGS3-silenced (BnSGS3-Si) oilseed rape (cv. Zhongshuang No. 6) plants. These three viruses are Oilseed rape mosaic virus (ORMV), Turnip mosaic virus (TuMV) and Cucumber mosaic virus (CMV). The native BnSGS3 expressed in all examined tissues with the highest expression in siliques. All three viruses induced BnSGS3 expression, but ORMV induced a dramatic increase in the BnSGS3-Ov plants, followed by TuMV and CMV. Upon inoculation with three different viruses, transcript abundance of BnSGS3 gene follows: BnSGS3-Ov > non-transgenic plants > BnSGS3-Si. The accumulation quantities of ORMV and TuMV exhibited a similar trend. However, CMV accumulation showed an opposite trend where virus accumulations were negatively correlated with BnSGS3 expression. The results suggest that BnSGS3 selectively inhibits CMV accumulation but promotes ORMV and TuMV accumulation. BnSGS3 should be used in different ways (up- and down-regulation) for breeding virus-resistant oilseed rape varieties. View Full-Text
Keywords: oilseed rape; virus; BnSGS3; virus accumulation; breeding strategy oilseed rape; virus; BnSGS3; virus accumulation; breeding strategy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Chen, Q.; Wang, J.; Hou, M.; Liu, S.; Huang, J.; Cai, L. BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape. Viruses 2015, 7, 4169-4185.

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