Next Article in Journal
RNase P Ribozymes Inhibit the Replication of Human Cytomegalovirus by Targeting Essential Viral Capsid Proteins
Next Article in Special Issue
Genome Characterization, Prevalence and Distribution of a Macula-Like Virus from Apis mellifera and Varroa destructor
Previous Article in Journal
Phylodynamics of H5N1 Highly Pathogenic Avian Influenza in Europe, 2005–2010: Potential for Molecular Surveillance of New Outbreaks
Previous Article in Special Issue
Honey Bee Infecting Lake Sinai Viruses
Article Menu

Export Article

Open AccessArticle
Viruses 2015, 7(6), 3329-3344; doi:10.3390/v7062774

Characterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry

1
ANSES, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France
2
Aix-Marseille Université, CNRS, AFMB UMR 7257, 13288 Marseille, France
3
Institute of Molecular and Cellular Pharmacology, IPMC, UMR6097 CNRS, 660 route des Lucioles, 06560 Valbonne, France
Deceased.
*
Author to whom correspondence should be addressed.
Academic Editors: Elke Genersch and Sebastian Gisder
Received: 25 March 2015 / Revised: 5 June 2015 / Accepted: 15 June 2015 / Published: 23 June 2015
(This article belongs to the Special Issue Honeybee Viruses)
View Full-Text   |   Download PDF [1116 KB, uploaded 25 June 2015]   |  

Abstract

Chronic bee paralysis virus (CBPV) is the etiological agent of chronic paralysis, an infectious and contagious disease in adult honeybees. CBPV is a positive single-stranded RNA virus which contains two major viral RNA fragments. RNA 1 (3674 nt) and RNA 2 (2305 nt) encode three and four putative open reading frames (ORFs), respectively. RNA 1 is thought to encode the viral RNA-dependent RNA polymerase (RdRp) since the amino acid sequence derived from ORF 3 shares similarities with the RdRP of families Nodaviridae and Tombusviridae. The genomic organization of CBPV and in silico analyses have suggested that RNA 1 encodes non-structural proteins, while RNA 2 encodes structural proteins, which are probably encoded by ORFs 2 and 3. In this study, purified CBPV particles were used to characterize virion proteins by mass spectrometry. Several polypeptides corresponding to proteins encoded by ORF 2 and 3 on RNA 2 were detected. Their role in the formation of the viral capsid is discussed. View Full-Text
Keywords: chronic bee paralysis virus (CBPV); nano-HPLC; MALDI-TOF/TOF; protein identification; hypothetical structural protein (hSP); predicted structural protein (pSP); anti-pSP antibodies chronic bee paralysis virus (CBPV); nano-HPLC; MALDI-TOF/TOF; protein identification; hypothetical structural protein (hSP); predicted structural protein (pSP); anti-pSP antibodies
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Chevin, A.; Coutard, B.; Blanchard, P.; Dabert-Gay, A.-S.; Ribière-Chabert, M.; Thiéry, R. Characterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry. Viruses 2015, 7, 3329-3344.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top