Next Article in Journal
Coordinated DNA Replication by the Bacteriophage T4 Replisome
Next Article in Special Issue
Honey Bee Infecting Lake Sinai Viruses
Previous Article in Journal
Antiviral Potential of a Novel Compound CW-33 against Enterovirus A71 via Inhibition of Viral 2A Protease
Previous Article in Special Issue
Dynamics of Apis mellifera Filamentous Virus (AmFV) Infections in Honey Bees and Relationships with Other Parasites
Article Menu

Export Article

Open AccessArticle
Viruses 2015, 7(6), 3172-3185; doi:10.3390/v7062765

The Effect of Oral Administration of dsRNA on Viral Replication and Mortality in Bombus terrestris

Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium
*
Author to whom correspondence should be addressed.
Academic Editors: Elke Genersch and Sebastian Gisder
Received: 31 March 2015 / Revised: 1 June 2015 / Accepted: 12 June 2015 / Published: 18 June 2015
(This article belongs to the Special Issue Honeybee Viruses)
View Full-Text   |   Download PDF [227 KB, uploaded 18 June 2015]   |  

Abstract

Israeli acute paralysis virus (IAPV), a single-stranded RNA virus, has a worldwide distribution and affects honeybees as well as other important pollinators. IAPV infection in honeybees has been successfully repressed by exploiting the RNA interference (RNAi) pathway of the insect’s innate immune response with virus-specific double stranded RNA (dsRNA). Here we investigated the effect of IAPV infection in the bumblebee Bombus terrestris and its tissue tropism. B. terrestris is a common pollinator of wild flowers in Europe and is used for biological pollination in agriculture. Infection experiments demonstrated a similar pathology and tissue tropism in bumblebees as reported for honeybees. The effect of oral administration of virus-specific dsRNA was examined and resulted in an effective silencing of the virus, irrespective of the length. Interestingly, we observed that non-specific dsRNA was also efficient against IAPV. However further study is needed to clarify the precise mechanism behind this effect. Finally we believe that our data are indicative of the possibility to use dsRNA for a broad range viral protection in bumblebees. View Full-Text
Keywords: IAPV; dsRNA; bumblebee; virus; immune response IAPV; dsRNA; bumblebee; virus; immune response
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Piot, N.; Snoeck, S.; Vanlede, M.; Smagghe, G.; Meeus, I. The Effect of Oral Administration of dsRNA on Viral Replication and Mortality in Bombus terrestris. Viruses 2015, 7, 3172-3185.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top