Next Article in Journal
Chimeric Rabies Virus-Like Particles Containing Membrane-Anchored GM-CSF Enhances the Immune Response against Rabies Virus
Previous Article in Journal
The Origin of the Variola Virus
Previous Article in Special Issue
Morbillivirus Infections: An Introduction
Article Menu

Export Article

Open AccessArticle
Viruses 2015, 7(3), 1113-1133; doi:10.3390/v7031113

Profiling of Measles-Specific Humoral Immunity in Individuals Following Two Doses of MMR Vaccine Using Proteome Microarrays

1
Mayo Vaccine Research Group, Mayo Clinic, Guggenheim 611C, 200 First Street SW, Rochester, MN 55905, USA
2
Program in Translational Immunovirology and Biodefense, Mayo Clinic and Foundation, Rochester, MN 55905, USA
3
Division of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN 55905, USA
4
Department of General Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Rik L. de Swart
Received: 18 December 2014 / Accepted: 20 February 2015 / Published: 10 March 2015
(This article belongs to the Special Issue Morbillivirus Infections)
View Full-Text   |   Download PDF [989 KB, uploaded 12 May 2015]   |  

Abstract

Introduction: Comprehensive evaluation of measles-specific humoral immunity after vaccination is important for determining new and/or additional correlates of vaccine immunogenicity and efficacy. Methods: We used a novel proteome microarray technology and statistical modeling to identify factors and models associated with measles-specific functional protective immunity in 150 measles vaccine recipients representing the extremes of neutralizing antibody response after two vaccine doses. Results: Our findings demonstrate a high seroprevalence of antibodies directed to the measles virus (MV) phosphoprotein (P), nucleoprotein (N), as well as antibodies to the large polymerase (L) protein (fragment 1234 to 1900 AA). Antibodies to these proteins, in addition to anti-F antibodies (and, to a lesser extent, anti-H antibodies), were correlated with neutralizing antibody titer and/or were associated with and predictive of neutralizing antibody response. Conclusion: Our results identify antibodies to specific measles virus proteins and statistical models for monitoring and assessment of measles-specific functional protective immunity in vaccinated individuals. View Full-Text
Keywords: measles; measles-mumps-rubella vaccine; measles virus; viral vaccines; antibodies; neutralizing; immunity; humoral; protein array analysis measles; measles-mumps-rubella vaccine; measles virus; viral vaccines; antibodies; neutralizing; immunity; humoral; protein array analysis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Haralambieva, I.H.; Simon, W.L.; Kennedy, R.B.; Ovsyannikova, I.G.; Warner, N.D.; Grill, D.E.; Poland, G.A. Profiling of Measles-Specific Humoral Immunity in Individuals Following Two Doses of MMR Vaccine Using Proteome Microarrays. Viruses 2015, 7, 1113-1133.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top