Next Article in Journal
Potential Broad Spectrum Inhibitors of the Coronavirus 3CLpro: A Virtual Screening and Structure-Based Drug Design Study
Next Article in Special Issue
A Flagellar Glycan-Specific Protein Encoded by Campylobacter Phages Inhibits Host Cell Growth
Previous Article in Journal
Innate Immunity and Immune Evasion by Enterovirus 71
Previous Article in Special Issue
What Can We Learn from a Metagenomic Analysis of a Georgian Bacteriophage Cocktail?
Article Menu

Export Article

Open AccessArticle
Viruses 2015, 7(12), 6631-6641; doi:10.3390/v7122962

Rapid Detection of Listeria by Bacteriophage Amplification and SERS-Lateral Flow Immunochromatography

Department of Chemistry and Geochemistry, Colorado School of Mines, Golden, CO 80401, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Abram Aertsen and Rob Lavigne
Received: 28 August 2015 / Revised: 2 November 2015 / Accepted: 10 December 2015 / Published: 14 December 2015
View Full-Text   |   Download PDF [4552 KB, uploaded 14 December 2015]   |  

Abstract

A rapid Listeria detection method was developed utilizing A511 bacteriophage amplification combined with surface-enhanced Raman spectroscopy (SERS) and lateral flow immunochromatography (LFI). Anti-A511 antibodies were covalently linked to SERS nanoparticles and printed onto nitrocellulose membranes. Antibody-conjugated SERS nanoparticles were used as quantifiable reporters. In the presence of A511, phage-SERS nanoparticle complexes were arrested and concentrated as a visible test line, which was interrogated quantitatively by Raman spectroscopy. An increase in SERS intensity correlated to an increase in captured phage-reporter complexes. SERS limit of detection was 6 × 106 pfu·mL−1, offering detection below that obtainable by the naked eye (LOD 6 × 107 pfu·mL−1). Phage amplification experiments were carried out at a multiplicity of infection (MOI) of 0.1 with 4 different starting phage concentrations monitored over time using SERS-LFI and validated by spot titer assay. Detection of L. monocytogenes concentrations of 1 × 107 colony forming units (cfu)·mL−1, 5 × 106 cfu·mL−1, 5 × 105 cfu·mL−1 and 5 × 104 cfu·mL−1 was achieved in 2, 2, 6, and 8 h, respectively. Similar experiments were conducted at a constant starting phage concentration (5 × 105 pfu·mL−1) with MOIs of 1, 2.5, and 5 and were detected in 2, 4, and 5 h, respectively. View Full-Text
Keywords: lateral flow immunochromatography; Listeria monocytogenes; surface-enhanced Raman spectroscopy; A511; phage amplification lateral flow immunochromatography; Listeria monocytogenes; surface-enhanced Raman spectroscopy; A511; phage amplification
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Stambach, N.R.; Carr, S.A.; Cox, C.R.; Voorhees, K.J. Rapid Detection of Listeria by Bacteriophage Amplification and SERS-Lateral Flow Immunochromatography. Viruses 2015, 7, 6631-6641.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top