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Viruses 2015, 7(11), 5844-5854; doi:10.3390/v7112908

Detection and Characterization of a Novel Reassortant Mammalian Orthoreovirus in Bats in Europe

1
Experimental Zooprophylactic Institute of Lombardy and Emilia-Romagna, Via Bianchi 9, 25124 Brescia, Italy
2
Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, SI-1000 Ljubljana, Slovenia
*
Author to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Received: 27 July 2015 / Revised: 26 October 2015 / Accepted: 3 November 2015 / Published: 11 November 2015
(This article belongs to the Section Animal Viruses)
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Abstract

A renewed interest in mammalian orthoreoviruses (MRVs) has emerged since new viruses related to bat MRV type 3, detected in Europe, were identified in humans and pigs with gastroenteritis. This study reports the isolation and characterization of a novel reassortant MRV from the lesser horseshoe bat (Rhinolophus hipposideros). The isolate, here designated BatMRV1-IT2011, was first identified by electron microscopy and confirmed using PCR and virus-neutralization tests. The full genome sequence was obtained by next-generation sequencing. Molecular and antigenic characterizations revealed that BatMRV1-IT2011 belonged to serotype 1, which had not previously been identified in bats. Phylogenetic and recombination detection program analyses suggested that BatMRV1-IT2011 was a reassortant strain containing an S1 genome segment similar to those of MRV T1/bovine/Maryland/Clone23/59 and C/bovine/ Indiana/MRV00304/2014, while other segments were more similar to MRVs of different hosts, origins and serotypes. The presence of neutralizing antibodies against MRVs has also been investigated in animals (dogs, pigs, bovines and horses). Preliminary results suggested that MRVs are widespread in animals and that infections containing multiple serotypes, including MRVs of serotype 1 with an S1 gene similar to BatMRV1-IT2011, are common. This paper extends the current knowledge of MRVs and stresses the importance to continue and improve MRV surveillance in bats and other mammals through the development and standardization of specific diagnostic tools. View Full-Text
Keywords: Orthoreovirus; bats; full-genome sequence; reassortment Orthoreovirus; bats; full-genome sequence; reassortment
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lelli, D.; Moreno, A.; Steyer, A.; Nagliˇc, T.; Chiapponi, C.; Prosperi, A.; Faccin, F.; Sozzi, E.; Lavazza, A. Detection and Characterization of a Novel Reassortant Mammalian Orthoreovirus in Bats in Europe. Viruses 2015, 7, 5844-5854.

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