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Viruses 2015, 7(10), 5632-5642; doi:10.3390/v7102896

Identification and Application of Neutralizing Epitopes of Human Adenovirus Type 55 Hexon Protein

1
State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510230, China
2
Dongguan Institute of Pediatrics, Dongguan Children’s Hospital, Dongguan 523325, China
3
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Received: 8 September 2015 / Revised: 10 October 2015 / Accepted: 20 October 2015 / Published: 27 October 2015
(This article belongs to the Section Animal Viruses)
View Full-Text   |   Download PDF [5332 KB, uploaded 27 October 2015]   |  

Abstract

Human adenovirus type 55 (HAdV55) is a newly identified re-emergent acute respiratory disease (ARD) pathogen with a proposed recombination of hexon gene between HAdV11 and HAdV14 strains. The identification of the neutralizing epitopes is important for the surveillance and vaccine development against HAdV55 infection. In this study, four type-specific epitope peptides of HAdV55 hexon protein, A55R1 (residues 138 to 152), A55R2 (residues 179 to 187), A55R4 (residues 247 to 259) and A55R7 (residues 429 to 443), were predicted by multiple sequence alignment and homology modeling methods, and then confirmed with synthetic peptides by enzyme-linked immunosorbent assay (ELISA) and neutralization tests (NT). Finally, the A55R2 was incorporated into human adenoviruses 3 (HAdV3) and a chimeric adenovirus rAd3A55R2 was successfully obtained. The chimeric rAd3A55R2 could induce neutralizing antibodies against both HAdV3 and HAdV55. This current study will contribute to the development of novel adenovirus vaccine candidate and adenovirus structural analysis. View Full-Text
Keywords: adenovirus type 55; neutralizing epitope; bivalent vaccine adenovirus type 55; neutralizing epitope; bivalent vaccine
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tian, X.; Ma, Q.; Jiang, Z.; Huang, J.; Liu, Q.; Lu, X.; Luo, Q.; Zhou, R. Identification and Application of Neutralizing Epitopes of Human Adenovirus Type 55 Hexon Protein. Viruses 2015, 7, 5632-5642.

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