Viruses 2014, 6(8), 3348-3362; doi:10.3390/v6083348
Review

Assembly and Maturation of a T = 4 Quasi-Equivalent Virus Is Guided by Electrostatic and Mechanical Forces

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Received: 4 June 2014; in revised form: 15 August 2014 / Accepted: 18 August 2014 / Published: 22 August 2014
(This article belongs to the Special Issue Virus Maturation)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Nudaurelia capensis w virus (NωV) is a eukaryotic RNA virus that is well suited for the study of virus maturation. The virus initially assembles at pH 7.6 into a marginally stable 480-Å procapsid formed by 240 copies of a single type of protein subunit. During maturation, which occurs during apoptosis at pH 5.0, electrostatic forces guide subunit trajectories into a robust 410-Å virion that is buttressed by subunit associated molecular switches. We discuss the competing factors in the virus capsid of requiring near-reversible interactions during initial assembly to avoid kinetic traps, while requiring robust stability to survive in the extra-cellular environment. In addition, viruses have a variety of mechanisms to deliver the genome, which must remain off while still inside the infected cell, yet turn on under the proper conditions of infection. We conclude that maturation is the process that provides a solution to these conflicting requirements through a program that is encoded in the procapsid and that leads to stability and infectivity.
Keywords: virus maturation; quasi-equivalence; Nudaurelia capensis omega virus; autoproteolysis; tetravirus; RNA insect virus; non-enveloped viruses
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MDPI and ACS Style

Kearney, B.M.; Johnson, J.E. Assembly and Maturation of a T = 4 Quasi-Equivalent Virus Is Guided by Electrostatic and Mechanical Forces. Viruses 2014, 6, 3348-3362.

AMA Style

Kearney BM, Johnson JE. Assembly and Maturation of a T = 4 Quasi-Equivalent Virus Is Guided by Electrostatic and Mechanical Forces. Viruses. 2014; 6(8):3348-3362.

Chicago/Turabian Style

Kearney, Bradley M.; Johnson, John E. 2014. "Assembly and Maturation of a T = 4 Quasi-Equivalent Virus Is Guided by Electrostatic and Mechanical Forces." Viruses 6, no. 8: 3348-3362.


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