Abstract: Influenza A virus is a successful parasite and requires host factors to complete its life cycle. Prop5 is an antisense oligonucleotide, targeting programmed cell death protein 5 (PDCD5). In this study, we tested the antiviral activity of prop5 against mouse-adapted A/FM/1/47 strain of influenza A virus in a mouse model. Prop5 intranasally administered the mice at dosages of 10 and 20 mg/kg/d at 24 h and 30 min before infection, provided 80% and 100% survival rates and prolonged mean survival days in comparison with influenza virus-infected mice (both p < 0.01). Moreover, viral titres in mice pretreated with prop5, at dose of 10 and 20 mg/kg/d, had declined significantly on day two, four, and six post-infection compared with the yields in infected mice (p < 0.05 or p < 0.01); lung index in mice pretreated with prop5 (20 mg/kg/d) had been inhibited on day six post-infection (p < 0.05). Western blotting and immunohistochemistry showed that prop5 could down-regulate the PDCD5 protein expression levels in lung tissues of infected mice. These data indicate that antisense oligonucleotide prop5 is a promising drug for prophylaxis and control influenza virus infections and provides an insight into the host-pathogen interaction.
Keywords: influenza A virus; host factor; antisense oligonucleotide; prop5
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Li, K.; Zhou, Z.; Wang, Y.O.; Liu, J.; Zhao, H.B.; Yang, J.; Wang, S.Q. Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections. Viruses 2014, 6, 573-581.
Li K, Zhou Z, Wang YO, Liu J, Zhao HB, Yang J, Wang SQ. Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections. Viruses. 2014; 6(2):573-581.
Li, Kang; Zhou, Zhe; Wang, Yu O.; Liu, Juan; Zhao, Hai B.; Yang, Jing; Wang, Sheng Q. 2014. "Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections." Viruses 6, no. 2: 573-581.