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Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections
Beijing University of Technology, No.100, Pingleyuan, Chaoyang District, Beijing 100124, China
Beijing Institute of Radiation Medicine, No.27, Taiping Road, Haidian District, Beijing 100850, China
Henan University of Traditional Chinese Medicine, No.1 Jinshui Road, Zhengzhou, Henan 450008, China
* Author to whom correspondence should be addressed.
Received: 9 September 2013; in revised form: 5 December 2013 / Accepted: 23 January 2014 / Published: 6 February 2014
Abstract: Influenza A virus is a successful parasite and requires host factors to complete its life cycle. Prop5 is an antisense oligonucleotide, targeting programmed cell death protein 5 (PDCD5). In this study, we tested the antiviral activity of prop5 against mouse-adapted A/FM/1/47 strain of influenza A virus in a mouse model. Prop5 intranasally administered the mice at dosages of 10 and 20 mg/kg/d at 24 h and 30 min before infection, provided 80% and 100% survival rates and prolonged mean survival days in comparison with influenza virus-infected mice (both p < 0.01). Moreover, viral titres in mice pretreated with prop5, at dose of 10 and 20 mg/kg/d, had declined significantly on day two, four, and six post-infection compared with the yields in infected mice (p < 0.05 or p < 0.01); lung index in mice pretreated with prop5 (20 mg/kg/d) had been inhibited on day six post-infection (p < 0.05). Western blotting and immunohistochemistry showed that prop5 could down-regulate the PDCD5 protein expression levels in lung tissues of infected mice. These data indicate that antisense oligonucleotide prop5 is a promising drug for prophylaxis and control influenza virus infections and provides an insight into the host-pathogen interaction.
Keywords: influenza A virus; host factor; antisense oligonucleotide; prop5
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MDPI and ACS Style
Li, K.; Zhou, Z.; Wang, Y.O.; Liu, J.; Zhao, H.B.; Yang, J.; Wang, S.Q. Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections. Viruses 2014, 6, 573-581.
Li K, Zhou Z, Wang YO, Liu J, Zhao HB, Yang J, Wang SQ. Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections. Viruses. 2014; 6(2):573-581.
Li, Kang; Zhou, Zhe; Wang, Yu O.; Liu, Juan; Zhao, Hai B.; Yang, Jing; Wang, Sheng Q. 2014. "Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections." Viruses 6, no. 2: 573-581.