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Viruses 2014, 6(2), 573-581; doi:10.3390/v6020573
Communication

Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections

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1 Beijing University of Technology, No.100, Pingleyuan, Chaoyang District, Beijing 100124, China 2 Beijing Institute of Radiation Medicine, No.27, Taiping Road, Haidian District, Beijing 100850, China 3 Henan University of Traditional Chinese Medicine, No.1 Jinshui Road, Zhengzhou, Henan 450008, China
* Author to whom correspondence should be addressed.
Received: 9 September 2013 / Revised: 5 December 2013 / Accepted: 23 January 2014 / Published: 6 February 2014
(This article belongs to the Section Antivirals & Vaccines)
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Abstract

Influenza A virus is a successful parasite and requires host factors to complete its life cycle. Prop5 is an antisense oligonucleotide, targeting programmed cell death protein 5 (PDCD5). In this study, we tested the antiviral activity of prop5 against mouse-adapted A/FM/1/47 strain of influenza A virus in a mouse model. Prop5 intranasally administered the mice at dosages of 10 and 20 mg/kg/d at 24 h and 30 min before infection, provided 80% and 100% survival rates and prolonged mean survival days in comparison with influenza virus-infected mice (both p < 0.01). Moreover, viral titres in mice pretreated with prop5, at dose of 10 and 20 mg/kg/d, had declined significantly on day two, four, and six post-infection compared with the yields in infected mice (p < 0.05 or p < 0.01); lung index in mice pretreated with prop5 (20 mg/kg/d) had been inhibited on day six post-infection (p < 0.05). Western blotting and immunohistochemistry showed that prop5 could down-regulate the PDCD5 protein expression levels in lung tissues of infected mice. These data indicate that antisense oligonucleotide prop5 is a promising drug for prophylaxis and control influenza virus infections and provides an insight into the host-pathogen interaction.
Keywords: influenza A virus; host factor; antisense oligonucleotide; prop5 influenza A virus; host factor; antisense oligonucleotide; prop5
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Li, K.; Zhou, Z.; Wang, Y.O.; Liu, J.; Zhao, H.B.; Yang, J.; Wang, S.Q. Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections. Viruses 2014, 6, 573-581.

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