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Viruses 2014, 6(1), 223-242; doi:10.3390/v6010223

Functional Characterization of a Bidirectional Plant Promoter from Cotton Leaf Curl Burewala Virus Using an Agrobacterium-Mediated Transient Assay

Plant Biotechnology Laboratory, Centre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore 53700, Pakistan
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Received: 14 October 2013 / Revised: 14 December 2013 / Accepted: 24 December 2013 / Published: 14 January 2014
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Abstract

The C1 promoter expressing the AC1 gene, and V1 promoter expressing the AV1 gene are located in opposite orientations in the large intergenic region of the Cotton leaf curl Burewala virus (CLCuBuV) genome. Agro-infiltration was used to transiently express putative promoter constructs in Nicotiana tabacum and Gossypium hirsutum leaves, which was monitored by a GUS reporter gene, and revealed that the bidirectional promoter of CLCuBuV transcriptionally regulates both the AC1 and AV1 genes. The CLCuBuV C1 gene promoter showed a strong, consistent transient expression of the reporter gene (GUS) in N. tabacum and G. hirsutum leaves and exhibited GUS activity two- to three-fold higher than the CaMV 35S promoter. The CLCuBuV bidirectional gene promoter is a nearly constitutive promoter that contains basic conserved elements. Many cis-regulatory elements (CREs) were also analyzed within the bidirectional plant promoters of CLCuBuV and closely related geminiviruses, which may be helpful in understanding the transcriptional regulation of both the virus and host plant. View Full-Text
Keywords: cotton leaf curl Burewala virus; agro-infiltration; bidirectional promoter; cis-regulatory elements; geminivirus cotton leaf curl Burewala virus; agro-infiltration; bidirectional promoter; cis-regulatory elements; geminivirus
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ashraf, M.A.; Shahid, A.A.; Rao, A.Q.; Bajwa, K.S.; Husnain, T. Functional Characterization of a Bidirectional Plant Promoter from Cotton Leaf Curl Burewala Virus Using an Agrobacterium-Mediated Transient Assay. Viruses 2014, 6, 223-242.

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