Next Article in Journal
Previous Article in Journal
Viruses 2014, 6(1), 223-242; doi:10.3390/v6010223
Article

Functional Characterization of a Bidirectional Plant Promoter from Cotton Leaf Curl Burewala Virus Using an Agrobacterium-Mediated Transient Assay

* ,
,
,
 and
Received: 14 October 2013; in revised form: 14 December 2013 / Accepted: 24 December 2013 / Published: 14 January 2014
(This article belongs to the Section Viruses of Plants, Fungi and Protoza)
View Full-Text   |   Download PDF [2650 KB, uploaded 14 January 2014]
Abstract: The C1 promoter expressing the AC1 gene, and V1 promoter expressing the AV1 gene are located in opposite orientations in the large intergenic region of the Cotton leaf curl Burewala virus (CLCuBuV) genome. Agro-infiltration was used to transiently express putative promoter constructs in Nicotiana tabacum and Gossypium hirsutum leaves, which was monitored by a GUS reporter gene, and revealed that the bidirectional promoter of CLCuBuV transcriptionally regulates both the AC1 and AV1 genes. The CLCuBuV C1 gene promoter showed a strong, consistent transient expression of the reporter gene (GUS) in N. tabacum and G. hirsutum leaves and exhibited GUS activity two- to three-fold higher than the CaMV 35S promoter. The CLCuBuV bidirectional gene promoter is a nearly constitutive promoter that contains basic conserved elements. Many cis-regulatory elements (CREs) were also analyzed within the bidirectional plant promoters of CLCuBuV and closely related geminiviruses, which may be helpful in understanding the transcriptional regulation of both the virus and host plant.
Keywords: cotton leaf curl Burewala virus; agro-infiltration; bidirectional promoter; cis-regulatory elements; geminivirus cotton leaf curl Burewala virus; agro-infiltration; bidirectional promoter; cis-regulatory elements; geminivirus
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Ashraf, M.A.; Shahid, A.A.; Rao, A.Q.; Bajwa, K.S.; Husnain, T. Functional Characterization of a Bidirectional Plant Promoter from Cotton Leaf Curl Burewala Virus Using an Agrobacterium-Mediated Transient Assay. Viruses 2014, 6, 223-242.

AMA Style

Ashraf MA, Shahid AA, Rao AQ, Bajwa KS, Husnain T. Functional Characterization of a Bidirectional Plant Promoter from Cotton Leaf Curl Burewala Virus Using an Agrobacterium-Mediated Transient Assay. Viruses. 2014; 6(1):223-242.

Chicago/Turabian Style

Ashraf, Muhammad A.; Shahid, Ahmad A.; Rao, Abdul Q.; Bajwa, Kamran S.; Husnain, Tayyab. 2014. "Functional Characterization of a Bidirectional Plant Promoter from Cotton Leaf Curl Burewala Virus Using an Agrobacterium-Mediated Transient Assay." Viruses 6, no. 1: 223-242.



Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert