Viruses 2013, 5(6), 1500-1515; doi:10.3390/v5061500
Review

The Role of Chromatin in Adenoviral Vector Function

Received: 12 April 2013; in revised form: 1 June 2013 / Accepted: 4 June 2013 / Published: 14 June 2013
(This article belongs to the Special Issue Chromatin Control of Viral Infection)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Vectors based on adenovirus (Ad) are one of the most commonly utilized platforms for gene delivery to cells in molecular biology studies and in gene therapy applications. Ad is also the most popular vector system in human clinical gene therapy trials, largely due to its advantageous characteristics such as high cloning capacity (up to 36 kb), ability to infect a wide variety of cell types and tissues, and relative safety due to it remaining episomal in transduced cells. The latest generation of Ad vectors, helper‑dependent Ad (hdAd), which are devoid of all viral protein coding sequences, can mediate high-level expression of a transgene for years in a variety of species ranging from rodents to non-human primates. Given the importance of histones and chromatin in modulating gene expression within the host cell, it is not surprising that Ad, a nuclear virus, also utilizes these proteins to protect the genome and modulate virus- or vector‑encoded genes. In this review, we will discuss our current understanding of the contribution of chromatin to Ad vector function.
Keywords: adenovirus; chromatin; histones; DNA replication; transcription
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MDPI and ACS Style

Wong, C.M.; McFall, E.R.; Burns, J.K.; Parks, R.J. The Role of Chromatin in Adenoviral Vector Function. Viruses 2013, 5, 1500-1515.

AMA Style

Wong CM, McFall ER, Burns JK, Parks RJ. The Role of Chromatin in Adenoviral Vector Function. Viruses. 2013; 5(6):1500-1515.

Chicago/Turabian Style

Wong, Carmen M.; McFall, Emily R.; Burns, Joseph K.; Parks, Robin J. 2013. "The Role of Chromatin in Adenoviral Vector Function." Viruses 5, no. 6: 1500-1515.

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