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Lost in Transcription: Molecular Mechanisms that Control HIV Latency
Viruses 2013, 5(4), 1143-1152; doi:10.3390/v5041143
Article

Induced Degradation of Tat by Nucleocapsid (NC) via the Proteasome Pathway and Its Effect on HIV Transcription

1
, 2
 and 1,*
Received: 18 March 2013; in revised form: 16 April 2013 / Accepted: 16 April 2013 / Published: 23 April 2013
(This article belongs to the Special Issue HIV Latency)
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Abstract: Human Immunodeficiency Virus type 1 (HIV-1) is a retrovirus that causes acquired immunodeficiency syndrome (AIDS). HIV-1 Tat protein upregulates transcriptional transactivation. The nucleocapsid protein NC of HIV-1 is a component of virion and plays a key role in genome packaging. Herein, we have demonstrated the interaction between NC and Tat by means of a yeast two-hybrid assay, GST pull-down analysis, co-immunoprecipitation and subcellular colocalization analysis. We observed that the level of Tat was significantly reduced in the presence of NC. But NC did not affect mRNA expression level of Tat. The level of Tat in the presence of NC was increased by treating cells with a proteasome inhibitor, MG132. The ubiquitination state of Tat was not seen to increase in the presence of NC, suggesting the proteasomal degradation was independent of ubiquitination. Lowered level of Tat in the presence of NC led to a decrease in Tat-mediated transcriptional transactivation.
Keywords: human immunodeficiency virus (HIV); nucleocapsid (NC); Tat; proteasomal degradation human immunodeficiency virus (HIV); nucleocapsid (NC); Tat; proteasomal degradation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Hong, H.-W.; Lee, S.-W.; Myung, H. Induced Degradation of Tat by Nucleocapsid (NC) via the Proteasome Pathway and Its Effect on HIV Transcription. Viruses 2013, 5, 1143-1152.

AMA Style

Hong H-W, Lee S-W, Myung H. Induced Degradation of Tat by Nucleocapsid (NC) via the Proteasome Pathway and Its Effect on HIV Transcription. Viruses. 2013; 5(4):1143-1152.

Chicago/Turabian Style

Hong, Hye-Won; Lee, Seong-Wook; Myung, Heejoon. 2013. "Induced Degradation of Tat by Nucleocapsid (NC) via the Proteasome Pathway and Its Effect on HIV Transcription." Viruses 5, no. 4: 1143-1152.


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